Amplite® Lactose Quantitation Kit

Amplite® Lactose Quantitation Kit is a fluorimetric and colorimetric assay kit for quantification of lactose in serum, plasma, other body fluids, food, and growth media.

Detection Principle: Converts lactose to galactose and glucose, then measures galactose oxidation through a coupled fluorescent/colorimetric reaction.
Signal Response: Fluorescence and absorbance signals are directly proportional to the initial lactose concentration.
Workflow: Incubates at 37°C for 10–30 minutes in a microplate format. AAT Bioquest Documentation
Instrument Compatibility: Supports fluorescence detection at Ex/Em 540/590 nm and absorbance at 570 nm.

Lactose dose response was measured with the Amplite® Lactose Quantification Ion Kit in a 96-well solid black plate using Fluorimetric analysis. The measurement was performed with SpectraMax (GEMINIXPS from Molecular Devices) fluorescence plate reader.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
40008	100 Tests	Price

Overview

Lactose, a natural disaccharide formed by the condensation of galactose and glucose molecules, is a major sugar in the milk of most species, typically ranging from 2-8%. Following ingestion, the enzyme lactase hydrolyzes lactose into its constituent monosaccharides for absorption. In specific individuals, particularly infants, the absence of the necessary galactose-digesting enzyme leads to Galactosemia, a disorder characterized by symptoms such as enlarged liver, renal failure, cataracts, and brain damage. The Amplite® Lactose Quantitation Kit employs an enzymatic assay to determine lactose concentration, converting lactose into galactose and glucose. Subsequent oxidation of galactose yields a colorimetric (570 nm) and fluorometric (Ex/Em = 540/590 nm) product, directly proportional to the initial lactose level. This versatile kit enables lactose measurement in diverse biological samples, including serum, plasma, other body fluids, food, and growth media. For researchers and clinicians, the kit proves invaluable, facilitating the assessment of lactose metabolism and lactose-related health implications across different biological contexts.

References

View all 13 references: Citation Explorer

Lactose Concentration in Low-Lactose and Lactose-Free Milk, Milk Products, and Products Containing Dairy Ingredients by High Sensitivity Enzymatic Method (K-LOLAC), Collaborative Study: Final Action 2020.08.

Authors:

Ivory, Ruth and Mangan, David and McCleary, Barry V

Journal:

Journal of AOAC International (2022): 1617-1624

Determination of Lactose Concentration in Low-Lactose and Lactose-Free Milk, Milk Products, and Products Containing Dairy Ingredients, Enzymatic Method: Single-Laboratory Validation First Action Method 2020.08.

Authors:

Ivory, Ruth and Delaney, Elaine and Mangan, David and McCleary, Barry V

Journal:

Journal of AOAC International (2021): 1308-1322

Adapting blood glucose meter biosensors for the measurement of lactose in dairy ingredients.

Authors:

Wagner, C and Amamcharla, J K and Rao, A and Metzger, L E

Journal:

Journal of dairy science (2020): 7585-7597

Enzymatic assay for urine lactose in the assessment of recent intravenous abuse of buprenorphine.

Authors:

Keltanen, Terhi N and Heikman, Pertti K and Muhonen, Leea H and Gunnar, Teemu O and Ojanperä, Ilkka A

Journal:

Drug testing and analysis (2019): 1412-1418

Cloning, Expression and Characterization of a Novel Cold-adapted β-galactosidase from the Deep-sea Bacterium Alteromonas sp. ML52.

Authors:

Sun, Jingjing and Yao, Congyu and Wang, Wei and Zhuang, Zhiwei and Liu, Junzhong and Dai, Fangqun and Hao, Jianhua

Journal:

Marine drugs (2018)

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	570 nm
Recommended plate	Clear bottom

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 25, 2026

Component A: Amplite® Red	1 vial
Component B: Assay Buffer	1 bottle (10 mL)
Component C: Horseradish Peroxidase	1 vial
Component D: Enzyme Mix	1 vial
Component E: DMSO	1 vial
Component F: Lactose Standard	1 vial