Amplite® Luciferase Reporter Gene Assay Kit *Maximized Luminescence*

Amplite® Luciferase Reporter Gene Assay Kit

Maximized Luminescence

Amplite® Luciferase Reporter Gene Assay Kit *Maximized Luminescence* is a chemiluminescent assay kit for quantification of luciferase activity in live cells and cell extracts.

Detection Principle: Measures ATP-dependent firefly luciferase oxidation of luciferin to generate bioluminescence.
Signal Advantage: Generates strong, sustained luminescence suitable for assays requiring high sensitivity.
Workflow: Optimized mix-and-read assay compatible with HTS liquid handling instruments.
Instrument Compatibility: Luminescence detection using a microplate reader with solid white plates.

<p>Reaction Kinetics of CHO-V<sub>2</sub>R-Luc cells using Amplite® Luciferase Reporter Gene Assay Kit. CHO cells stably transfected with pCRE-luciferase gene and human Vasopressin receptor 2 (V<sub>2</sub>R) were plated into a 384-well white wall/clear bottom costar plate at 15,000 cells/well/25 µL. Cells then were treated with 100 nM of vasopressin in a 5% CO<sub>2</sub> incubator at 37 °C for 4 hours. 25 µL of luciferase assay solution was added into the well. The kinetic data was taken every 30 minutes for up to 3 hours with a NOVOstar plate reader (BMG Labtech). The vasopressin induced luciferase signal is stable for more than 3 hours.</p>

Protocol

Catalog	Size	Price
12518	1 plate	Price
12519	10 plates	Price
12520	100 plates	Price

Overview

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The advantages of a luciferase assay are the high sensitivity, the absence of luciferase activity inside most of the cell types, the wide dynamic range, rapidity and low cost. The most versatile and common reporter gene is the luciferase of the North American firefly Photinus pyralis. The protein requires no posttranslational modification for enzyme activity. It is not even toxic in high concentration (in vivo) and can be used in pro- and eukaryotic cells. The firefly luciferase catalyzes the bioluminescent oxidation of luciferin in the presence of ATP, magnesium and oxygen. This Amplite® Luciferase Reporter Gene Assay Kit uses a proprietary luminogenic formulation to quantify luciferase activity in live cells and cell extracts. Our formulation generates a luminescent product that gives strong luminescence upon interaction with luciferase. The kit provides all the essential components with our optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments. It has extremely high sensitivity, and can be used for the assays that require demanding sensitivity.

Citations

View all 12 citations: Citation Explorer

Comprehensive profiling of metal compound toxicity: potential novel targets and mechanistic insights using Tox21 screening

Authors:

Ooka, Masato and Adesina, Precious and Goyal, Vishakha and Zhao, Jinghua and Li, Shuaizhang and Feldman, Gabrielle and TeKrony, Charlotte and Blivis, Dvir and Harouni, Majid and Hall, Matthew D and others,

Journal:

Journal of Hazardous Materials Advances (2026): 101063

Use of Tox21 screening data to profile PFAS bioactivities on nuclear receptors, cellular stress pathways, and cytochrome p450 enzymes

Authors:

Ooka, Masato and Sakamuru, Srilatha and Zhao, Jinghua and Qu, Yanyan and Fang, Yuhong and Tao, Dingyin and Huang, Ruili and Ferguson, Stephen and Reif, David and Simeonov, Anton and others,

Journal:

Journal of Hazardous Materials (2024): 134642

SARS-CoV-2 omicron variants harbor spike protein mutations responsible for their attenuated fusogenic phenotype

Authors:

Park, Seung Bum and Khan, Mohsin and Chiliveri, Sai Chaitanya and Hu, Xin and Irvin, Parker and Leek, Madeleine and Grieshaber, Ailis and Hu, Zongyi and Jang, Eun Sun and Bax, Ad and others,

Journal:

Communications Biology (2023): 556

Discovery of small molecule agonists of the Relaxin Family Peptide Receptor 2

Authors:

Esteban-Lopez, Maria and Wilson, Kenneth J and Myhr, Courtney and Kaftanovskaya, Elena M and Henderson, Mark J and Southall, Noel T and Xu, Xin and Wang, Amy and Hu, Xin and Barnaeva, Elena and others,

Journal:

Communications biology (2022): 1--12

AtHSPR is involved in GA-and light intensity-mediated control of flowering time and seed set in Arabidopsis

Authors:

Yang, Tao and Sun, Yan and Wang, Yongli and Zhou, Lina and Chen, Mengya and Bian, Zhiyuan and Lian, Yuke and Xuan, Lijuan and Yuan, Guoqiang and Wang, Xinyu and others,

Journal:

Journal of experimental botany (2020): 3543--3559

References

View all 52 references: Citation Explorer

Non-invasive imaging of firefly luciferase reporter gene expression using bioluminescence imaging in human prostate cancer models

Authors:

Li H, Li JZ, Helm GA, Pan D.

Journal:

Biotechnol Appl Biochem (2007): 179

Adaptation of a luciferase gene reporter and lac expression system to Borrelia burgdorferi

Authors:

Blevins JS, Revel AT, Smith AH, Bachlani GN, Norgard MV.

Journal:

Appl Environ Microbiol (2007): 1501

Functional characterization of cell lines for high-throughput screening of human neuromedin U receptor subtype 2 specific agonists using a luciferase reporter gene assay

Authors:

Li X, Shen F, Zhang Y, Zhu J, Huang L, Shi Q.

Journal:

Eur J Pharm Biopharm (2007): 284

A GUS/luciferase fusion reporter for plant gene trapping and for assay of promoter activity with luciferin-dependent control of the reporter protein stability

Authors:

Koo J, Kim Y, Kim J, Yeom M, Lee IC, Nam HG.

Journal:

Plant Cell Physiol (2007): 1121

Reporter gene assay against lipophilic chemicals based on site-specific genomic recombination of a nuclear receptor gene, its response element, and a luciferase reporter gene within a stable HeLa cell line

Authors:

Mori T, Saito F, Yoshino T, Takeyama H, Matsunaga T.

Journal:

Biotechnol Bioeng. (2007)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Luminescence microplate reader
Recommended plate	Solid white

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Page updated on October 14, 2026

Component A: Luciferase Sensor (Light-sensitive)	1 bottle
Component B: Assay Buffer	1 bottle (10 mL)

Component A: Luciferase Sensor	1 bottle
Component B: Assay Buffer	1 bottle (100 mL)

Component A: Luciferase Sensor	1 bottle
Component B: Assay Buffer	1 bottle (1000 mL)