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Amplite® Luciferase Reporter Gene Assay Kit
Maximized Luminescence
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The advantages of a luciferase assay are the high sensitivity, the absence of luciferase activity inside most of the cell types, the wide dynamic range, rapidity and low cost. The most versatile and common reporter gene is the luciferase of the North American firefly Photinus pyralis. The protein requires no posttranslational modification for enzyme activity. It is not even toxic in high concentration (in vivo) and can be used in pro- and eukaryotic cells. The firefly luciferase catalyzes the bioluminescent oxidation of luciferin in the presence of ATP, magnesium and oxygen. This Amplite® Luciferase Reporter Gene Assay Kit uses a proprietary luminogenic formulation to quantify luciferase activity in live cells and cell extracts. Our formulation generates a luminescent product that gives strong luminescence upon interaction with luciferase. The kit provides all the essential components with our optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments. It has extremely high sensitivity, and can be used for the assays that require demanding sensitivity.
<p>Reaction Kinetics of CHO-V<sub>2</sub>R-Luc cells using Amplite® Luciferase Reporter Gene Assay Kit. CHO cells stably transfected with pCRE-luciferase gene and human Vasopressin receptor 2 (V<sub>2</sub>R) were plated into a 384-well white wall/clear bottom costar plate at 15,000 cells/well/25 &micro;L. Cells then were treated with 100 nM of vasopressin in a 5% CO<sub>2</sub> incubator at 37 &deg;C for 4 hours. 25 &micro;L of luciferase assay solution was added into the well. The kinetic data was taken every 30 minutes for up to 3 hours with a NOVOstar plate reader (BMG Labtech). The vasopressin induced luciferase signal is stable for more than 3 hours.</p>
<p>Reaction Kinetics of CHO-V<sub>2</sub>R-Luc cells using Amplite® Luciferase Reporter Gene Assay Kit. CHO cells stably transfected with pCRE-luciferase gene and human Vasopressin receptor 2 (V<sub>2</sub>R) were plated into a 384-well white wall/clear bottom costar plate at 15,000 cells/well/25 &micro;L. Cells then were treated with 100 nM of vasopressin in a 5% CO<sub>2</sub> incubator at 37 &deg;C for 4 hours. 25 &micro;L of luciferase assay solution was added into the well. The kinetic data was taken every 30 minutes for up to 3 hours with a NOVOstar plate reader (BMG Labtech). The vasopressin induced luciferase signal is stable for more than 3 hours.</p>
protocol
Protocol
COA
CatalogSize
Price
Quantity
125181 plate
Price
1251910 plates
Price
12520100 plates
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC41105331
Instrument settings
Luminescence microplate reader
Recommended plateSolid white
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Page updated on October 13, 2025