Name: Amplite® Luminometric Alkaline Phosphatase Assay Kit *Luminescence*
Brand: AAT Bioquest
SKU: 11956
Price: 232 USD
Availability: InStock

Amplite® Luminometric Alkaline Phosphatase Assay Kit

Luminescence

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications. It is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. This Amplite® Alkaline Phosphatase Assay Kit uses a proprietary luminogenic phosphatase substrate, to quantify alkaline phosphatase activity in solutions as well as in cell extracts. This proprietary phosphatase substrate generates a luminescent product that produces strong luminescence upon interaction with phosphatase. The kit provides all the essential components with our optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments. It has extremely high sensitivity, and can be used for the assays that require demanding sensitivity.

Alkaline phosphatase dose response was measured with Amplite® Luminometric Alkaline Phosphatase Assay Kit in a white 96-well plate using a NovoStar microplate reader (BMG Labtech). As low as 0.001 mU/mL alkaline phosphatase can be detected with 20 minutes incubation (n=3).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11956	100 Tests	Price

Citations

View all 9 citations: Citation Explorer

The impact of various scaffold components on vascularized bone constructs

Authors:

Eweida, Ahmad and Schulte, Matthias and Frisch, Oliver and Kneser, Ulrich and Harhaus, Leila

Journal:

Journal of Cranio-Maxillofacial Surgery (2017)

A Mineralized High Strength and Tough Hydrogel for Skull Bone Regeneration

Authors:

Xu, Bing and Zheng, Pengbin and Gao, Fei and Wang, Wei and Zhang, Hongtao and Zhang, Xuran and Feng, Xuequan and Liu, Wenguang

Journal:

Advanced Functional Materials (2016)

DRG axon elongation and growth cone collapse rate induced by Sema3A are differently dependent on NGF concentration

Authors:

Kaselis, Andrius and Treinys, Rimantas and Vosyliute, Ruta and Satkauskas, Saulius

Journal:

Cellular and molecular neurobiology (2014): 289--296

Acute oral toxicity and kinetic behaviors of inorganic layered nanoparticles

Authors:

Yu, Jin and Chung, Hea-Eun and Choi, Soo-Jin

Journal:

Journal of Nanomaterials (2013): 12

Effect of Some Antihypertensive Drugs on Alkaline Phosphatase and DNA of Mice

Authors:

El-Khawaga, OY and El-Waseef, A and Ellazec, YO and El-Naggar, MM and Alla, Abd M

Journal:

International Journal of Genomics and Proteomics (2013): 60

References

View all 109 references: Citation Explorer

8-Quinolyl phosphate as a substrate for the fluorimetric determination of alkaline phosphatase

Authors:

Zhu X, Jiang C.

Journal:

Clin Chim Acta. (2006)

Effects of hydrogen peroxide (H(2)O(2)) on alkaline phosphatase activity and matrix mineralization of odontoblast and osteoblast cell lines

Authors:

Lee DH, Lim BS, Lee YK, Yang HC.

Journal:

Cell Biol Toxicol (2006): 39

The effect of alkaline phosphatase inhibitors on intracellular lipid accumulation in preadipocytes isolated from human mammary tissue

Authors:

Ali AT, Penny CB, Paiker JE, Psaras G, Ikram F, Crowther NJ.

Journal:

Ann Clin Biochem (2006): 207

Alkaline phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1

Authors:

Ali AT, Penny CB, Paiker JE, van Niekerk C, Smit A, Ferris WF, Crowther NJ.

Journal:

Clin Chim Acta (2005): 101

Insertion of GPI-anchored alkaline phosphatase into supported membranes: a combined AFM and fluorescence microscopy study

Authors:

Rieu JP, Ronzon F, Place C, Dekkiche F, Cross B, Roux B.

Journal:

Acta Biochim Pol (2004): 189

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Luminescence microplate reader
Recommended plate	Solid white

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Page updated on October 6, 2025

Component A: Phosphatase Substrate	1 vial (lyophilized powder)
Component B: Reaction Buffer	1 bottle (5 mL)
Component C: Alkaline Phosphatase Standard	1 vial (lyophilized powder, 10 units)
Component D: Assay Buffer	1 bottle (5 mL)