Name: Amplite® Luminometric Nitroreductase Assay Kit
Brand: AAT Bioquest
SKU: 12470
Price: 355 USD
Availability: InStock

Amplite® Luminometric Nitroreductase Assay Kit

Amplite® Luminometric Nitroreductase Assay Kit is a chemiluminescent assay kit for quantification of nitroreductase activity in a microplate format.

Detection Principle: Uses an NTR-reducible luciferin derivative, with generated luciferin measured by a luciferase detection system.
Sensitivity: Detects nitroreductase down to 20 ng/mL.
Workflow: Incubates the NTR reaction for 60 minutes at 37°C before luminescence detection.
Instrument Compatibility: Luminescence detection using a microplate reader with solid white plates.

Nitroreductase dose response was measured in a white solid 96-well plate with Amplite® Luminometric Nitroreductase Assay Kit using with a NOVO star plate reader (BMG Labtech). The kit can detect as low as 20 ng/mL of NTR (The data was obtained about 5 min after adding the NTR detection working solution).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12470	100 Tests	Price

Overview

Nitroreductases (NTR) are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds. They are absent in mammalian cells but widespread in bacteria. Nitroreductases play a crucial role in the reduction of nitroaromatic compounds via NAD(P)H-dependent reactions. NTR are also targets for developing novel antibiotics, degradation of pollutants, and cancer therapy. Although the quantification of NTR becomes extremely important for a number of biological applications, most of the current NTR assays are based on ELISA format with low sensitivity and low throughput. Amplite®™ Luminometric Nitroreductase Assay Kit provides a highly sensitive and convenient method to quantify NTR activity. The kit uses a luciferin derivative that can be selectively reduced by NTR luciferin. The amount of generated luciferin is conveniently detected with the well-known luciferase detection system. The luminescent intensity generated is proportional to the NTR activity. This luminometric nitroreductase assay kit has been formulated to have minimal hands-on time and stable luminescence signal. It can detect as low as 20 ng/mL of NTR.

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Luminescence microplate reader
Recommended plate	Solid white

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Page updated on October 23, 2026

Component A: NTR Substrate	1 vial
Component B: NTR Reaction Buffer	25 mL
Component C: NTR Reaction Enzyme	1 vial
Component D: NTR Detection Buffer	5 mL
Component E: NTR Detection Mix 1	1 vial
Component F: NTR Detection Mix 2	1 vial
Component G: Nitroreductase Standard	1 vial
Component H: DMSO	100 uL