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Amplite® Luminometric Nitroreductase Assay Kit
Nitroreductases (NTR) are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds. They are absent in mammalian cells but widespread in bacteria. Nitroreductases play a crucial role in the reduction of nitroaromatic compounds via NAD(P)H-dependent reactions. NTR are also targets for developing novel antibiotics, degradation of pollutants, and cancer therapy. Although the quantification of NTR becomes extremely important for a number of biological applications, most of the current NTR assays are based on ELISA format with low sensitivity and low throughput. Amplite®™ Luminometric Nitroreductase Assay Kit provides a highly sensitive and convenient method to quantify NTR activity. The kit uses a luciferin derivative that can be selectively reduced by NTR luciferin. The amount of generated luciferin is conveniently detected with the well-known luciferase detection system. The luminescent intensity generated is proportional to the NTR activity. This luminometric nitroreductase assay kit has been formulated to have minimal hands-on time and stable luminescence signal. It can detect as low as 20 ng/mL of NTR.
Nitroreductase dose response was measured in a white solid 96-well plate with Amplite® Luminometric Nitroreductase Assay Kit using with a NOVO star plate reader (BMG Labtech). The kit can detect as low as 20 ng/mL of NTR (The data was obtained about 5 min after adding the NTR detection working solution).
Nitroreductase dose response was measured in a white solid 96-well plate with Amplite® Luminometric Nitroreductase Assay Kit using with a NOVO star plate reader (BMG Labtech). The kit can detect as low as 20 ng/mL of NTR (The data was obtained about 5 min after adding the NTR detection working solution).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
12470100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Luminescence microplate reader
Recommended plateSolid white
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Page updated on October 13, 2025