Name: Amplite® Luminometric Peroxidase (HRP) Assay Kit
Brand: AAT Bioquest
SKU: 11559
Price: 237 USD
Availability: InStock

Amplite® Luminometric Peroxidase (HRP) Assay Kit

Amplite® Luminometric Peroxidase (HRP) Assay Kit is a chemiluminescent assay kit for detection of horseradish peroxidase activity in a microplate format.

Detection Principle: Measures HRP activity through an H₂O₂-dependent luminescent reaction.
Workflow: One-step, homogeneous, no-wash mix-and-read assay.
Instrument Compatibility: Luminescence detection using a microplate reader with solid white plates.
Applications: Supports ELISAs, enzyme kinetics studies, and high-throughput screening of oxidase inhibitors.

HRP dose response was measured with Amplite® Luminometric Peroxidase Assay Kit in a solid black 384-well plate using a NOVOstar plate reader (BMG Labtech).

Protocol

SDS

Catalog	Size	Price	Quantity
11559	500 Tests	Price

Overview

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio. Due to its small size, it rarely causes steric hindrance problem with antibody/antigen complex formation. Peroxidase is inexpensive compared to other labeling enzymes. The major disadvantage associated with peroxidase is their low tolerance to many preservatives such as sodium azide that inactivates peroxidase activity even at low concentration. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques and Northern, Southern and Western blot analyses. We offer this quick (10 min) HRP assay in a one-step, homogeneous, no wash assay system. The kit can be used for ELISAs, characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors, etc. The kit provides an optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.

References

View all 49 references: Citation Explorer

Horseradish peroxidase-driven fluorescent labeling of nanotubes with quantum dots

Authors:

Didenko VV, Baskin DS.

Journal:

Biotechniques (2006): 295

Enzymatic oxidation of dipyridamole in homogeneous and micellar solutions in the horseradish peroxidase-hydrogen peroxide system

Authors:

Almeida LE, Imasato H, Tabak M.

Journal:

Biochim Biophys Acta (2006): 216

Recent advances in catalytic peroxidase histochemistry

Authors:

Krieg R, Halbhuber KJ.

Journal:

Cell Mol Biol (Noisy-le-grand) (2003): 547

Vesicular transport route of horseradish C1a peroxidase is regulated by N- and C-terminal propeptides in tobacco cells

Authors:

Matsui T, Nakayama H, Yoshida K, Shinmyo A.

Journal:

Appl Microbiol Biotechnol (2003): 517

Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization

Authors:

van Gijlswijk RP, van de Corput MP, Bezrookove V, Wiegant J, Tanke HJ, Raap AK.

Journal:

Histochem Cell Biol (2000): 175

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Luminescence microplate reader
Recommended plate	Solid white

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Assay Buffer	1 bottle (25 mL)
Component B: H2O2	1 vial (3% stabilized solution, 200 µL)
Component C: Horseradish Peroxidase	1 vial (20 units)