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Amplite® Luminometric Peroxidase (HRP) Assay Kit
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio. Due to its small size, it rarely causes steric hindrance problem with antibody/antigen complex formation. Peroxidase is inexpensive compared to other labeling enzymes. The major disadvantage associated with peroxidase is their low tolerance to many preservatives such as sodium azide that inactivates peroxidase activity even at low concentration. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques and Northern, Southern and Western blot analyses. We offer this quick (10 min) HRP assay in a one-step, homogeneous, no wash assay system. The kit can be used for ELISAs, characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors, etc. The kit provides an optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.
HRP dose response was measured with Amplite® Luminometric Peroxidase Assay Kit in a solid black 384-well plate using a NOVOstar plate reader (BMG Labtech).
HRP dose response was measured with Amplite® Luminometric Peroxidase Assay Kit in a solid black 384-well plate using a NOVOstar plate reader (BMG Labtech).
CatalogSize
Price
Quantity
11559500 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings

Luminescence microplate reader
Recommended plateSolid white
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Page updated on September 25, 2025