Amplite® MMP-3 Activity Assay Kit *Green Fluorescence*

Amplite® MMP-3 Activity Assay Kit

Green Fluorescence

Amplite® MMP-3 Activity Assay Kit is a fluorimetric assay kit for detection of MMP-3 activity in biological samples.

Detection Principle: MMP-3 cleavage of the MMP-3 Green™ FRET peptide separates the TF2/TQ2 pair and increases fluorescence.
Specificity: The FRET substrate is designed for relatively high selectivity toward MMP-3.
Workflow: Supports kinetic or endpoint measurement; endpoint assays incubate for 30–60 minutes after substrate addition.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/525 nm using solid black microplates.

Dose response of MMP-3 enzyme activity was measured with Amplite® MMP-3 Activity Assay Kit using a NOVOStar microplate reader (BMG Labtech).

Protocol

SDS

Catalog	Size	Price	Quantity
13512	100 Tests	Price

Overview

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix. These enzymes are responsible for the breakdown of connective tissues and are important in bone remodeling, the menstrual cycle and repair of tissue damage. While the exact contribution of MMPs to certain pathological processes is difficult to assess, MMPs appear to play a key role in the development of arthritis as well as in the invasion and metastasis of cancer. MMPs tend to have multiple substrates, with most family members having the ability to degrade different types of collagen along with elastin, gelatin and fibronectin. It is quite difficult to find a substrate that is selective to a single MMP enzyme. This FRET substrate is designed to have a relative high selectivity to MMP-3. It is used to monitor the MMP-3 activity. It can also be used to screening MMP-3 inhibitors when a purified MMP-3 enzyme is used. This FRET substrate is based on our TF2/TQ2 FRET pair. Upon MMP-3 hydrolysis the fluorescence of MMP-3 Green™ FRET peptide substrate is increased since the TF2/TQ2 FRET pair is separated. The fluorescence increase is proportional to the MMP-3 enzyme activities.

Citations

View all 12 citations: Citation Explorer

Nanodevice-Mediated Immune Cell Recruitment: Targeting Senescent Cells via MMP-3-Responsive CXCL12-Coated Nanoparticles

Authors:

Escriche-Navarro, Blanca and Garrido, Eva and Clara-Trujillo, Sandra and Labernadie, Anna and Sancenon, F{\'e}lix and Garc{\'\i}a-Fern{\'a}ndez, Alba and Mart{\'\i}nez-M{\'a}{\~n}ez, Ram{\'o}n

Journal:

ACS Applied Materials \& Interfaces (2025)

Decrease in the Ratio proBDNF/BDNF in the Urine of Aging Female Patients with OAB

Authors:

Covarrubias, Claudia and Cammisotto, Philippe G and Shamout, Samer and Campeau, Lysanne

Journal:

Metabolites (2023): 723

Zinc ions regulate opening of tight junction favouring efflux of macromolecules via the GSK3β/snail-mediated pathway

Authors:

Xiao, Ruyue and Yuan, Lan and He, Weijiang and Yang, Xiaoda

Journal:

Metallomics (2018)

Probing Cell Adhesion Profiles with a Microscale Adhesive Choice Assay

Authors:

Kittur, Harsha and Tay, Andy and Hua, Avery and Yu, Min and Di Carlo, Dino

Journal:

Biophysical Journal (2017): 1858--1867

DACT2, an epigenetic stimulator, exerts dual efficacy for colorectal cancer prevention and treatment

Authors:

Lu, Linlin and Wang, Ying and Ou, Rilan and Feng, Qian and Ji, Liyan and Zheng, Hongming and Guo, Yue and Qi, Xiaoxiao and Kong, Ah-Ng Tony and Liu, Zhongqiu

Journal:

Pharmacological research (2017)

Spectral properties

Excitation (nm)	494
Emission (nm)	515

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 28, 2026

Component A: MMP-3 Green™ substrate Solution	1 vial (60 µL)
Component B: APMA	1 vial (20 µL, 1 M)
Component C: Assay Buffer	1 bottle (20 mL)