Name: Amplite® Rapid Colorimetric Maleimide Quantitation Kit
Brand: AAT Bioquest
SKU: 5526
Price: 237 USD
Availability: InStock

Amplite® Rapid Colorimetric Maleimide Quantitation Kit

Amplite® Rapid Colorimetric Maleimide Quantitation Kit is a colorimetric assay kit for quantification of maleimide groups on proteins, oligonucleotides, and nucleic acids.

Detection Principle: Quantifies maleimide by reacting samples with Maleimide Blue™ and measuring the purified product spectrophotometrically.
Quantitation Method: Calculates maleimide-to-sample ratios from absorbance at 780 nm relative to 280 nm for proteins or 260 nm for oligos and nucleic acids.
Workflow: Uses a single spin-column purification step to remove excess sensor before measurement.
Instrument Compatibility: Compatible with traditional cuvettes, NanoDrop™ spectrophotometers, and 96-well absorbance plate readers using UV-transparent plates.

<p>The Amplite® Rapid Colorimetric Maleimide Quantitation Assay Principle.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5526	2 Tests	Price

Overview

A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins, or proteins to other biomolecules. A key challenge in maleimide crosslinking technology has been the quantitation of maleimide linked to a single protein or antibody. Maleimides can be directly assayed spectrophotometrically at 302 nm. However, the small extinction coefficient of 620 M-1cm-1 renders this assay insensitive, and the assay is further complicated by the protein absorbance at the same wavelength. AAT Bioquest's Amplite® Rapid Colorimetric Maleimide Quantitation Kit provides a rapid and accurate method to quantify maleimide using our proprietary maleimide sensor Maleimide Blue™ with the maximum absorbance at ~780nm. The principle of this assay is that Maleimide Blue™ reacts with the maleimide-linked sample, and the resulted product is run through a single spin column to remove the excess sensor. The absorption spectrum of the purified product is measured, and the amount of maleimide to protein ratio can be calculated from the absorbance ratio of 780 nm and 280 nm (for proteins) or 260 nm (for oligos and nucleic acids). This Amplite® Rapid Maleimide Quantitation kit can be performed in a traditional cuvette, NanoDrop™ Spectrophotometer or a convenient 96-well absorbance plate reader with a UV-transparent plate. This kit has been widely used for the rapid quantification of maleimide group from protein, oligo and nucleic acid samples.

Citations

View all 6 citations: Citation Explorer

Conjugation with an inulin-chitosan adjuvant markedly improves the immunogenicity of Mycobacterium tuberculosis CFP10-TB10. 4 fusion protein

Authors:

Yu, Weili and Hu, Tao

Journal:

Molecular Pharmaceutics (2016)

Successful acquisition of a neutralizing monoclonal antibody against a novel neutrophil-activating peptide, mitocryptide-1

Authors:

Hattori, Tatsuya and Nakashima, Kenta and Marutani, Takayuki and Kiso, Yoshiaki and Nishi, Yoshisuke and Mukai, Hidehito

Journal:

Biochemical and biophysical research communications (2015): 54--59

Rapid quantification of maleimide in bioconjuated samples using a novel colorimetric method (TECH2P. 761)

Authors:

Liao, Jinfang and Guo, Haitao and Luo, Zhen and Zhao, Qin and Diwu, Jack

Journal:

The Journal of Immunology (2015): 206--7

Moderate PEGylation of the carrier protein improves the polysaccharide-specific immunogenicity of meningococcal group A polysaccharide conjugate vaccine

Authors:

Zhang, Tingting and Yu, Weili and Wang, Yanfei and Hu, Tao

Journal:

Vaccine (2015): 3208--3214

PEG as a spacer arm markedly increases the immunogenicity of meningococcal group Y polysaccharide conjugate vaccine

Authors:

Huang, Qingrui and Li, Dongxia and Kang, Aijun and An, Wenqi and Fan, Bei and Ma, Xiaowei and Ma, Guanghui and Su, Zhiguo and Hu, Tao

Journal:

Journal of Controlled Release (2013): 382--389

References

View all 38 references: Citation Explorer

Characterization of site-specific ScFv PEGylation for tumor-targeting pharmaceuticals

Authors:

Natarajan A, Xiong CY, Albrecht H, DeNardo GL, DeNardo SJ.

Journal:

Bioconjug Chem (2005): 113

A new maleimide-bound acid-cleavable solid-support reagent for profiling phosphorylation

Authors:

Chowdhury SM, Munske GR, Siems WF, Bruce JE.

Journal:

Rapid Commun Mass Spectrom (2005): 899

Quantitation of microparticles released from coated-platelets

Authors:

Dale GL, Remenyi G, Friese P.

Journal:

J Thromb Haemost (2005): 2081

Determination of WR-1065 in human blood by high-performance liquid chromatography following fluorescent derivatization by a maleimide reagent ThioGlo3

Authors:

Chen J, Lu Z, Lawrence TS, Smith DE.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2005): 161

Reactions of cysteines substituted in the amphipathic N-terminal tail of a bacterial potassium channel with hydrophilic and hydrophobic maleimides

Authors:

Li J, Xu Q, Cortes DM, Perozo E, Laskey A, Karlin A.

Journal:

Proc Natl Acad Sci U S A (2002): 11605

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	900 nm to 250 nm
Recommended plate	Clear bottom

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Email	sales@aatbio.com
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Page updated on October 28, 2026

Component A: Maleimide Blue™	2 vials (One vial is for 50 ~100 µg maleimidelinked antibody or protein)
Component B: Assay Buffer	1 bottle (15 mL)
Component C: Spin Column	2 columns
Component D: Washing Tube (2 mL)	2 tubes
Component E: Collecting Tube (1.5 mL)	2 tubes