Name: Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit
Brand: AAT Bioquest
SKU: 5530
Price: 237 USD
Availability: InStock

Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit

Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit is a colorimetric assay kit for quantification of maleimide groups on maleimide-activated PE proteins.

Detection Principle: Quantifies maleimide by reacting PE-maleimide with Maleimide 680™ and measuring the purified reaction product.
Quantitation Method: Determines the maleimide-to-PE ratio from absorbance at 680 nm and 566 nm.
Workflow: Uses a spin-column purification step to remove unreacted sensor before measurement.
Instrument Compatibility: Compatible with cuvettes, NanoDrop™ spectrophotometers, and 96-well absorbance plate readers.

Amplite® Rapid Colorimetric PE-Maleimide Quantitation Assay Principle.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5530	2 Tests	Price

Overview

Maleimide-based crosslinking reagents are commonly used for conjugating proteins to other proteins or biomolecules. A significant challenge in maleimide chemistry is the precise quantification of maleimide moieties conjugated to proteins. The Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm. The assay involves the reaction of Maleimide 680™ with the maleimide-activated PE, followed by the separation of the reaction mixture via a spin column to remove the unreacted sensor. Subsequently, the absorption spectrum of the isolated product is measured and the amount of maleimide to PE is determined from the absorbance ratios at 680 nm and 566 nm, with the latter being the peak absorbance of PE. This quantitation kit is compatible with various detection systems, including traditional cuvettes, NanoDrop™ Spectrophotometers, and 96-well absorbance plate readers. It offers a robust and adaptable approach for the rapid quantification of maleimide modifications on PE proteins, addressing a critical need in protein biochemistry.

References

View all 50 references: Citation Explorer

Antibody-dye Conjugates Targeting EGFR and HER2 for the Photoimmunotherapy of Bladder Cancer.

Authors:

Huber, Fabian and Wolf, Isis and Storz, Jonas and Schultze-Seemann, Susanne and Lauw, Susan and Klemenz, Lukas and Miernik, Arkadiusz and Gratzke, Christian and Brückner, Reinhard and Wolf, Philipp

Journal:

Anticancer research (2024): 1837-1844

The medicinal chemistry evolution of antibody-drug conjugates.

Authors:

Hobson, Adrian D

Journal:

RSC medicinal chemistry (2024): 809-831

Click chemistry in the synthesis of antibody-drug conjugates.

Authors:

Dudchak, Rostyslav and Podolak, Magdalena and Holota, Serhii and Szewczyk-Roszczenko, Olga and Roszczenko, Piotr and Bielawska, Anna and Lesyk, Roman and Bielawski, Krzysztof

Journal:

Bioorganic chemistry (2024): 106982

Enhanced therapeutic potential of antibody fragment via IEDDA-mediated site-specific albumin conjugation.

Authors:

Go, Eun Byeol and Lee, Jae Hun and Cho, Jeong Haeng and Kwon, Na Hyun and Choi, Jong-Il and Kwon, Inchan

Journal:

Journal of biological engineering (2024): 23

Linker substitution influences succinimide ring hydrolysis equilibrium impacting the stability of attachment to antibody-drug conjugates.

Authors:

Wang, Lu and Hobson, Adrian D and Salomon, Paulin L and Fitzgibbons, Julia and Xu, Jianwen and McCarthy, Sean and Wu, Kan and Jia, Ying and Hernandez, Axel and Li, Xiang and Xu, Zhou and Wang, Zhongyuan and Yu, Yajie and Li, Junxian and Tao, Lin

Journal:

RSC medicinal chemistry (2024): 612-622

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	900 nm to 250 nm
Recommended plate	Clear bottom

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Page updated on October 20, 2026

Component A: Maleimide 680™	2 Vials (1 vial is for 100 µg of PE-maleimide)
Component B: Assay Buffer	1 Bottle (15 mL)
Component C: Spin Column	2 Columns