Name: Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit
Brand: AAT Bioquest
SKU: 5536
Price: 218 USD
Availability: InStock

Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit

Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit is a colorimetric assay kit for quantification of aldehyde groups on aldehyde-modified proteins.

Detection Principle: Quantifies protein aldehyde content using absorbance measurements of the labeled protein product.
Quantitation Method: Determines the aldehyde-to-protein ratio from absorbance at 495 nm and 280 nm.
Workflow: Uses spin-column purification to remove excess labeling reagent before measurement.
Instrument Compatibility: Compatible with cuvettes, NanoDrop™ spectrophotometers, and 96-well absorbance plate readers.

Aldehyde quantitation of BSA-Acrolein Conjugate with Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit. Absorbance spctrum was measured with NanoDrop Spectrometer.<br />Aldehyde/BSA =  ((A495/ 75000) / [(A280 – 0.117 × A495)/ 43824] = 5.8

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5536	2 Tests	Price

Overview

Site-selective modification of proteins is of major interest in chemical biology. Most of the traditional methods used for protein modifications are based on reactions of amine or thiol groups within a protein. However, many methods have been developed to incorporate aldehyde functional groups into proteins. Rapid and accurate measurement of aldehyde content is an important tool for biological and chemical research. Amplite®™ Rapid Colorimetric Protein Aldehyde Content Quantitation Kit provides an accurate method to quantify aldehyde groups using our proprietary AldeView™ 488 sensor, which has the maximum absorbance at ~495nm. AldeView™ 488 reacts with the aldehyde-modified protein samples. The resulted product is run through a single spin column to remove the excess AldeView™ 488 sensor. The absorbance spectra of purified product are measured, and the aldehyde to protein ratio can be determined via the absorbance ratio of 495 nm/280 nm. Amplite®™ Rapid Colorimetric Protein Aldehyde Content Quantitation Kit can be performed in a traditional cuvette, NanoDrop™ Spectrophotometer or a convenient 96-well absorbance plate reader with a UV-transparent plate.

References

View all 21 references: Citation Explorer

Vacuolar targeting of aldehyde dehydrogenase 6 tagging with signal peptide of proteinase A.

Authors:

Park, Dong J and Choi, Wooil and Bang, Seung H and Kim, Sang Y and Wee, Ji-Hyang and Kim, Yang-Hoon and Min, Jiho

Journal:

Journal of basic microbiology (2020): 341-350

Palladium-unleashed proteins: gentle aldehyde decaging for site-selective protein modification.

Authors:

Brabham, Robin L and Spears, Richard J and Walton, Julia and Tyagi, Swati and Lemke, Edward A and Fascione, Martin A

Journal:

Chemical communications (Cambridge, England) (2018): 1501-1504

E. coli metabolic protein aldehyde-alcohol dehydrogenase-E binds to the ribosome: a unique moonlighting action revealed.

Authors:

Shasmal, Manidip and Dey, Sandip and Shaikh, Tanvir R and Bhakta, Sayan and Sengupta, Jayati

Journal:

Scientific reports (2016): 19936

Current state and use of biological adhesives in orthopedic surgery.

Authors:

Shah, Neil V and Meislin, Robert

Journal:

Orthopedics (2013): 945-56

Inhibition of CYP2E1 leads to decreased malondialdehyde-acetaldehyde adduct formation in VL-17A cells under chronic alcohol exposure.

Authors:

Swaminathan, Kavitha and Clemens, Dahn L and Dey, Aparajita

Journal:

Life sciences (2013): 325-36

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

NanoDrop
Absorbance	280 nm and 495 nm

Spectrophotometer
Absorbance	250-750 nm
Recommended plate	Cuvette

Absorbance microplate reader
Absorbance	280 nm and 495 nm
Recommended plate	Clear bottom

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Fax
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Page updated on October 24, 2026

Component A: AldeView™ 488	2 vials
Component B: Reaction Buffer	1 vial (30 μL)
Component C: Spin Column	2 columns