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Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit
Site-selective modification of proteins is of major interest in chemical biology. Most of the traditional methods used for protein modifications are based on reactions of amine or thiol groups within a protein. However, many methods have been developed to incorporate aldehyde functional groups into proteins. Rapid and accurate measurement of aldehyde content is an important tool for biological and chemical research. Amplite®™ Rapid Colorimetric Protein Aldehyde Content Quantitation Kit provides an accurate method to quantify aldehyde groups using our proprietary AldeView™ 488 sensor, which has the maximum absorbance at ~495nm. AldeView™ 488 reacts with the aldehyde-modified protein samples. The resulted product is run through a single spin column to remove the excess AldeView™ 488 sensor. The absorbance spectra of purified product are measured, and the aldehyde to protein ratio can be determined via the absorbance ratio of 495 nm/280 nm. Amplite®™ Rapid Colorimetric Protein Aldehyde Content Quantitation Kit can be performed in a traditional cuvette, NanoDrop™ Spectrophotometer or a convenient 96-well absorbance plate reader with a UV-transparent plate.
Aldehyde quantitation of BSA-Acrolein Conjugate with Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit. Absorbance spctrum was measured with NanoDrop Spectrometer.<br />Aldehyde/BSA =&nbsp; ((A495/ 75000) / [(A280 &ndash; 0.117 &times; A495)/ 43824] = 5.8
Aldehyde quantitation of BSA-Acrolein Conjugate with Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit. Absorbance spctrum was measured with NanoDrop Spectrometer.<br />Aldehyde/BSA =&nbsp; ((A495/ 75000) / [(A280 &ndash; 0.117 &times; A495)/ 43824] = 5.8
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
55362 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
NanoDrop
Absorbance280 nm and 495 nm
Spectrophotometer
Absorbance250-750 nm
Recommended plateCuvette
Absorbance microplate reader
Absorbance280 nm and 495 nm
Recommended plateClear bottom
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Page updated on October 12, 2025