Amplite® Renilla Luciferase Reporter Gene Assay Kit *Maximized Luminescence*

Amplite® Renilla Luciferase Reporter Gene Assay Kit

Maximized Luminescence

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The most versatile reporter gene is the firefly luciferase. Recently there is steadily increasing use of other luciferases, such as Renilla luciferase since these reporters are smaller and do not require the presence of ATP. Our Amplite® Renilla Luciferase Reporter Gene Assay Kit is designed to provide a fast and sensitive method to detect the luciferase from sea pansy (Renilla reniformis). It uses a proprietary luminogenic formulation to quantify Renilla luciferase activity in cell-based assays. Our formulation generates a luminescent product that gives strong luminescence upon interaction with Renilla luciferase. The kit provides all the essential components. It has high sensitivity and can be performed in a convenient 96-well and 384-well microtiter-plate format. The "glow-type" signal with a half-life of one hour provides a consistent signal across large number of assay plates. The assay is compatible with standard cell growth media. This kit enables the measurement of primary expression or gene expression with wild type and the synthetic hRluc genes .

Renilla Luciferase was measured with Amplite&trade; Renilla Luciferase Reporter Gene Assay Kit in a white 96-well plate with a NOVOstar plate reader (BMG Labtech).

Protocol

COA

Catalog	Size	Price
12535	1 plate	Price
12536	10 plates	Price
12537	100 plates	Price

References

View all 56 references: Citation Explorer

RNA detection using peptide-inserted Renilla luciferase

Authors:

Andou T, Endoh T, Mie M, Kobatake E.

Journal:

Anal Bioanal Chem (2009): 661

The cAMP-dependent protein kinase inhibitor H-89 attenuates the bioluminescence signal produced by Renilla Luciferase

Authors:

Herbst KJ, Allen MD, Zhang J.

Journal:

PLoS One (2009): e5642

Reassembly of a bioluminescent protein Renilla luciferase directed through DNA hybridization

Authors:

Cissell KA, Rahimi Y, Shrestha S, Deo SK.

Journal:

Bioconjug Chem (2009): 15

Bioluminescent indicators for Ca2+ based on split Renilla luciferase complementation in living cells

Authors:

Kaihara A, Umezawa Y, Furukawa T.

Journal:

Anal Sci (2008): 1405

Coelenterazine-binding protein of Renilla muelleri: cDNA cloning, overexpression, and characterization as a substrate of luciferase

Authors:

Titushin MS, Markova SV, Frank LA, Malikova NP, Stepanyuk GA, Lee J, Vysotski ES.

Journal:

Photochem Photobiol Sci (2008): 189

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	41105331

Instrument settings

Luminescence microplate reader
Recommended plate	Solid white

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Page updated on October 13, 2025

Component A: 100X Luciferase Substrate (Light-Sensitive)	1 vial (100 µL)
Component B: Assay Buffer	1 bottle (10 mL)

Component A: 100X Luciferase Substrate (Light-Sensitive)	1 vial (1 mL)
Component B: Assay Buffer	1 bottle (100 mL)

Component A: 100X Luciferase Substrate (Light-Sensitive)	10 x 1 mL/vial
Component B: Assay Buffer	10 x100 mL/bottle