Amplite® Renilla Luciferase Reporter Gene Assay Kit *Maximized Luminescence*

Amplite® Renilla Luciferase Reporter Gene Assay Kit

Maximized Luminescence

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Maximized Luminescence* is a chemiluminescent assay kit for quantification of Renilla luciferase activity in cell-based assays.

Detection Principle: Measures Renilla luciferase using a proprietary luminogenic formulation that generates light upon enzyme interaction.
Signal Advantage: Produces a glow-type signal with an approximately 1-hour half-life for consistent measurements across multiple plates.
Plate Format: Compatible with 96- and 384-well microplates.
Instrument Compatibility: Uses a luminescence microplate reader with solid white plates recommended for detection.

Renilla Luciferase was measured with Amplite&trade; Renilla Luciferase Reporter Gene Assay Kit in a white 96-well plate with a NOVOstar plate reader (BMG Labtech).

Protocol

Catalog	Size	Price
12535	1 plate	Price
12536	10 plates	Price
12537	100 plates	Price

Overview

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The most versatile reporter gene is the firefly luciferase. Recently there is steadily increasing use of other luciferases, such as Renilla luciferase since these reporters are smaller and do not require the presence of ATP. Our Amplite® Renilla Luciferase Reporter Gene Assay Kit is designed to provide a fast and sensitive method to detect the luciferase from sea pansy (Renilla reniformis). It uses a proprietary luminogenic formulation to quantify Renilla luciferase activity in cell-based assays. Our formulation generates a luminescent product that gives strong luminescence upon interaction with Renilla luciferase. The kit provides all the essential components. It has high sensitivity and can be performed in a convenient 96-well and 384-well microtiter-plate format. The "glow-type" signal with a half-life of one hour provides a consistent signal across large number of assay plates. The assay is compatible with standard cell growth media. This kit enables the measurement of primary expression or gene expression with wild type and the synthetic hRluc genes.

References

View all 56 references: Citation Explorer

RNA detection using peptide-inserted Renilla luciferase

Authors:

Andou T, Endoh T, Mie M, Kobatake E.

Journal:

Anal Bioanal Chem (2009): 661

The cAMP-dependent protein kinase inhibitor H-89 attenuates the bioluminescence signal produced by Renilla Luciferase

Authors:

Herbst KJ, Allen MD, Zhang J.

Journal:

PLoS One (2009): e5642

Reassembly of a bioluminescent protein Renilla luciferase directed through DNA hybridization

Authors:

Cissell KA, Rahimi Y, Shrestha S, Deo SK.

Journal:

Bioconjug Chem (2009): 15

Bioluminescent indicators for Ca2+ based on split Renilla luciferase complementation in living cells

Authors:

Kaihara A, Umezawa Y, Furukawa T.

Journal:

Anal Sci (2008): 1405

Coelenterazine-binding protein of Renilla muelleri: cDNA cloning, overexpression, and characterization as a substrate of luciferase

Authors:

Titushin MS, Markova SV, Frank LA, Malikova NP, Stepanyuk GA, Lee J, Vysotski ES.

Journal:

Photochem Photobiol Sci (2008): 189

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Luminescence microplate reader
Recommended plate	Solid white

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Page updated on October 20, 2026

Component A: 100X Luciferase Substrate (Light-Sensitive)	1 vial (100 µL)
Component B: Assay Buffer	1 bottle (10 mL)

Component A: 100X Luciferase Substrate	1 vial (1 mL)
Component B: Assay Buffer	1 bottle (100 mL)

Component A: 100X Luciferase Substrate	10 x 1 mL/vial
Component B: Assay Buffer	10 x100 mL/bottle