Name: Amplite® Total Alcohol Quantification Assay Kit
Brand: AAT Bioquest
SKU: 40003
Price: 403 USD
Availability: InStock

Amplite® Total Alcohol Quantification Assay Kit

Amplite® Total Alcohol Quantification Assay Kit enables accurate, high-throughput detection of alcohols across multiple research and quality control workflows.

High sensitivity and robustness: Detects low levels of alcohol with reliable performance
Dual-mode readout: Compatible with both fluorescence and absorbance plate readers
Rapid and scalable protocol: Mix-and-read format completes within 30 minutes, ideal for high-throughput setups
Widely applicable alternative: Provides a dependable, non-radioactive method for total alcohol measurement across diverse sample types

Ethanol content was measured with Amplite® Total Alcohol Quantitation Assay Kit (Cat# 40003) on a solid black 96-well plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
40003	100 tests	Price

Overview

Amplite® Total Alcohol Quantification Assay Kit is a fast, non-radioactive assay designed for the sensitive detection of total alcohol content in biological samples. It uses a proprietary reaction detected with Amplite® Red, a fluorogenic and chromogenic substrate that allows versatile signal measurement.

The kit supports both fluorimetric and colorimetric detection modes, allowing flexible integration into diverse assay platforms. With a simple mix-and-read protocol, the assay is completed in under 30 minutes and is easily scalable for high-throughput applications. It is ideal for life science research, clinical evaluations, and total alcohol quantitation in food and beverage testing.

References

View all 49 references: Citation Explorer

High-throughput quantitation of acetaldehyde and ethanol in mice using gas chromatography/mass spectrometry positive chemical ionization.

Authors:

Lin, Yu-Hong and Chen, Cheng and Wei, Shoupeng and Adrien, Guillot and Mackowiak, Bryan and Pan, Hongna and Fu, Yaojie and Maccioni, Luca and Ren, Tianyi and Zhang, Li and Hibbeln, Joseph and Pawlosky, Robert and Gao, Bin

Journal:

Alcohol, clinical & experimental research (2025): 1897-1911

Development and Validation of a Rapid Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantitation of Vitamin K Metabolites in Different Matrices.

Authors:

Wang, Danchen and Ma, Yichen and Li, Honglei and Ma, Xiaoli and Zou, Yutong and Yu, Songlin and Qiu, Ling

Journal:

Journal of mass spectrometry : JMS (2025): e5120

Comparison of approaches for assessing detection and quantitation limits in bioanalytical methods using HPLC for sotalol in plasma.

Authors:

Lambarki, Lamia Zaari and Jhilal, Fayssal and Slimani, Lamia and El Hajji, Ridouan and Bakkali, Fadil and Iskandar, Samy and El Jemli, Mariam and Ihssane, Bouchaib and El Ghali, Wafaa and Saffaj, Taoufiq

Journal:

Scientific reports (2025): 5472

Quantitation of alcohols and acetone in postmortem blood and urine using headspace gas chromatography mass spectrometry.

Authors:

Edstam, Monika and Sundqvist, Gustav and Kronstrand, Robert

Journal:

Journal of analytical toxicology (2025)

The P(III)-Amidite Based Synthesis of Stable Isotope Labeled mRNA-Cap-Structures Enables their Sensitive Quantitation from Brain Tissue.

Authors:

Ripp, Alexander and Krämer, Martina and Barth, Vanessa and Moser, Patrick and Haas, Thomas M and Singh, Jyoti and Huck, Tamara and Gleue, Lukas and Friedland, Kristina and Helm, Mark and Jessen, Henning J

Journal:

Angewandte Chemie (International ed. in English) (2025): e202414537

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	576 ± 5 nm
Recommended plate	Clear bottom

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on July 15, 2026

Component A: Amplite® Total Alcohol Reagent (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (10 mL)
Component C: Alcohol Enzyme Mix (lyophilized)	1 vial
Component D: DMSO	1 vial (200 µL)
Component E: Ethanol Standard	(100%, 0.5 mL)