Name: Amplite® Total Sulfide Quantification Kit
Brand: AAT Bioquest
SKU: 21300
Price: 339 USD
Availability: InStock

Amplite® Total Sulfide Quantification Kit

Amplite® Total Sulfide Quantification Kit is a fluorimetric assay kit for detection of sulfide anion in solution.

Detection Principle: Sulfide Green™ generates green fluorescence proportional to sulfide concentration.
Quantitation Range: Supports low-range 1–100 µM and high-range 100–10,000 µM sulfide measurements.
Workflow: Supports 96- or 384-well formats and can be adapted to automation with no separation step.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/530 nm using a microplate reader.

Low sulfide dose (1~100 uM) response was measured with Amplite® Sulfide Quantification Kit in a 96-well solid black plate.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
21300	100 Tests	Price

Overview

Hydrogen sulfide is not usually a health risk at concentrations present in household water. Water containing hydrogen sulfide, commonly called sulfur water, has a distinctive "rotten egg" odor, which may be especially noticeable when running hot water. Such water can discolor coffee, tea and other beverages, and alter the appearance and taste of cooked foods. However, hydrogen sulfide dissolved in water can corrode plumbing metals, such as iron, steel, copper and brass and exposed metal parts in washing machines and other water-using appliances. The corrosion of iron and steel from hydrogen sulfide forms ferrous sulfide or "black water" which can darken silverware and discolor copper and brass utensils. Hydrogen sulfide can also interfere with the effectiveness of water softeners. High sulfide concentration is poisonous. Amplite® Sulfide Quantification Kit provides a robust method for detecting sulfide anion in solution. It uses Sulfide Green™, a selective and sensitive green sulfide probe that can be easily monitored with a fluorescence microplate reader (Ex/Em = 490/530 nm). Upon reaction with sulfide anion, Sulfide Green generates strong green fluorescence that is proportional to sulfide concentration. Amplite® Fluorimetric Sulfide Anion Quantitation Kit can be performed in a convenient 96-well or 384-well microplate format and easily adapted to automation with no separation steps required. The assay can be completed within 30 minutes.

References

View all 50 references: Citation Explorer

Simultaneous biological nitrification and desulfurization treatment of ammonium and sulfide-rich wastewater: Effectiveness of a sequential batch operation.

Authors:

Sekine, Mutsumi and Akizuki, Shinichi and Kishi, Masatoshi and Kurosawa, Norio and Toda, Tatsuki

Journal:

Chemosphere (2020): 125381

Global airborne sampling reveals a previously unobserved dimethyl sulfide oxidation mechanism in the marine atmosphere.

Authors:

Veres, Patrick R and Neuman, J Andrew and Bertram, Timothy H and Assaf, Emmanuel and Wolfe, Glenn M and Williamson, Christina J and Weinzierl, Bernadett and Tilmes, Simone and Thompson, Chelsea R and Thames, Alexander B and Schroder, Jason C and Saiz-Lopez, Alfonso and Rollins, Andrew W and Roberts, James M and Price, Derek and Peischl, Jeff and Nault, Benjamin A and Møller, Kristian H and Miller, David O and Meinardi, Simone and Li, Qinyi and Lamarque, Jean-François and Kupc, Agnieszka and Kjaergaard, Henrik G and Kinnison, Douglas and Jimenez, Jose L and Jernigan, Christopher M and Hornbrook, Rebecca S and Hills, Alan and Dollner, Maximilian and Day, Douglas A and Cuevas, Carlos A and Campuzano-Jost, Pedro and Burkholder, James and Bui, T Paul and Brune, William H and Brown, Steven S and Brock, Charles A and Bourgeois, Ilann and Blake, Donald R and Apel, Eric C and Ryerson, Thomas B

Journal:

Proceedings of the National Academy of Sciences of the United States of America (2020)

Therapeutic importance of hydrogen sulfide in age-associated neurodegenerative diseases.

Authors:

Tabassum, Rubaiya and Jeong, Na Young and Jung, Junyang

Journal:

Neural regeneration research (2020): 653-662

Controlled-synthesis of alumina-graphene oxide nanocomposite coupled with DNA/ sulfide fluorophore for eco-friendly "Turn off/on" H2S nanobiosensor.

Authors:

Samak, Nadia A and Selim, Mohamed S and Hao, Zhifeng and Xing, Jianmin

Journal:

Talanta (2020): 120655

A new colorimetric DET technique for determining mm-resolution sulfide porewater distributions and allowing improved interpretation of iron(II) co-distributions.

Authors:

Kankanamge, Nadeeka Rathnayake and Bennett, William W and Teasdale, Peter R and Huang, Jianyin and Welsh, David T

Journal:

Chemosphere (2020): 125388

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	530 nm
Cutoff	515 nm
Recommended plate	Solid black
Instrument specification(s)	Top read mode

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Email	sales@aatbio.com
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Page updated on October 23, 2026

Component A: Sulfide Green™	1 vial
Component B: Sulfide Assay Buffer	1 bottle (12.5 mL)
Component C: Sulfide Standard (50 mM)	1 vial (0.2 mL)
Component D: DMSO	1 vial (100 μL)