Tyramide signal amplification (TSA) is a technique used to enhance the signal of target molecules, such as antibodies or nucleic acid probes, in immunohistochemistry and in situ hybridization. This technique is particularly useful when working with samples that have low levels of target molecules or weak signals. TSA works by utilizing the enzymatic activity of horseradish peroxidase (HRP), which can catalyze the deposition of labeled tyramide molecules in close proximity to the target molecules. The tyramide molecules contain a reporter group, such as a fluorescent dye, which generates a detectable signal upon enzymatic reaction. This localized deposition of labeled tyramide molecules leads to signal amplification at the site of the target molecules, making them more easily detectable. There are a number of fluorescent tyramides available for TSA applications. However, some of the commercial tyramide conjugates have low signals. Amplite® Tyramide (TSA) Signal Booster is used to further enhance the TSA signal. It has been validated to enhance the TSA signal of tyramides prepared from Alexa Fluor® 350, 430, 450, 488, 514, 546, 568 and 594, Cy3, rhodamines, fluoresceins and coumarins.