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Amplite® Universal Fluorimetric Kinase Assay Kit
Red Fluorescence
Most of commercial protein kinase assay kits are either based on monitoring of phosphopeptide formation or ATP deletion. For the kinase assay kits that are based on detection of phosphopeptides one has to spend time and efforts to identify an optimized peptide substrate while the ATP depletion method suffers various interferences due to the use of luciferase that are inhibited or activated by various biological compounds. The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.
ADP dose response was measured with Amplite® Universal Fluorimetric Kinase Assay Kit in a solid black 384-well plate using a Gemini fluorescence microplate reader (Molecular Devices).
ADP dose response was measured with Amplite® Universal Fluorimetric Kinase Assay Kit in a solid black 384-well plate using a Gemini fluorescence microplate reader (Molecular Devices).
CatalogSize
Price
Quantity
31001250 Tests
Price
31002500 Tests
Price
 
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on October 8, 2025