Name: Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 31001
Price: 465 USD
Availability: InStock

Amplite® Universal Fluorimetric Kinase Assay Kit

Red Fluorescence

Most of commercial protein kinase assay kits are either based on monitoring of phosphopeptide formation or ATP deletion. For the kinase assay kits that are based on detection of phosphopeptides one has to spend time and efforts to identify an optimized peptide substrate while the ATP depletion method suffers various interferences due to the use of luciferase that are inhibited or activated by various biological compounds. The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.

ADP dose response was measured with Amplite® Universal Fluorimetric Kinase Assay Kit in a solid black 384-well plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

Catalog	Size	Price	Quantity
31001	250 Tests	Price
31002	500 Tests	Price

Components

Catalog 31001 (250 Tests)

Component A: ADP Sensor Buffer	1 vial (5 mL)
Component B1: ADP Sensor I (Light-sensitive)	1 vial powder
Component B2: ADP Sensor II	1 vial (2.5 mL)
Component B3: DMSO	1 vial (100 µL)
Component C: ADP Standard	1 vial
Component D: ADP Assay Buffer	1 vial (10 mL)

Catalog 31002 (500 Tests)

Component A: ADP Sensor Buffer	Discontinued
Component B1: ADP Sensor I (Light-sensitive)	Discontinued
Component B2: ADP Sensor II	Discontinued
Component B3: DMSO	Discontinued
Component C: ADP Standard	Discontinued
Component D: ADP Assay Buffer	Discontinued

Citations

View all 12 citations: Citation Explorer

Ribosomal modification protein rimK-like family member A activates betaine-homocysteine S-methyltransferase 1 to ameliorate hepatic steatosis

Authors:

Yan, Han and Liu, Wenjun and Xiang, Rui and Li, Xin and Hou, Song and Xu, Luzheng and Wang, Lin and Zhao, Dong and Liu, Xingkai and Wang, Guoqing and others,

Journal:

Signal Transduction and Targeted Therapy (2024): 214

Resveratrol-induced Sirt1 phosphorylation by LKB1 mediates mitochondrial metabolism

Authors:

Huang, Yuanyuan and Lu, Jianlin and Zhan, Li and Wang, Ming and Shi, Ronghua and Yuan, Xiao and Gao, Xinjiao and Liu, Xing and Zang, Jianye and Liu, Wei and others,

Journal:

Journal of Biological Chemistry (2021)

Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregation

Authors:

Xu, Leilei and Ali, Mahboob and Duan, Wenxiu and Yuan, Xiao and Garba, Fatima and Mullen, McKay and Sun, Binwen and Poser, Ina and Duan, Hequan and Lu, Jianlin and others,

Journal:

Cell Reports (2021): 109343

Methylation of PLK1 by SET7/9 ensures accurate kinetochore--microtubule dynamics

Authors:

Yu, Ruoying and Wu, Huihui and Ismail, Hazrat and Du, Shihao and Cao, Jun and Wang, Jianyu and Ward, Tarsha and Yang, Fengrui and Gui, Ping and Ali, Mahboob and others,

Journal:

Journal of molecular cell biology (2020): 462--476

Dynamic acetylation of the kinetochore-associated protein HEC1 ensures accurate microtubule--kinetochore attachment

Authors:

Zhao, Gangyin and Cheng, Yubao and Gui, Ping and Cui, Meiying and Liu, Wei and Wang, Wenwen and Wang, Xueying and Ali, Mahboob and Dou, Zhen and Niu, Liwen and others,

Journal:

Journal of Biological Chemistry (2019): 576--592

References

View all 30 references: Citation Explorer

Fluorescence detection techniques for protein kinase assay

Authors:

Li Y, Xie W, Fang G.

Journal:

Anal Bioanal Chem (2008): 2049

A multiplexed protein kinase assay

Authors:

Shults MD, Kozlov IA, Nelson N, Kermani BG, Melnyk PC, Shevchenko V, Srinivasan A, Musmacker J, Hachmann JP, Barker DL, Lebl M, Zhao C.

Journal:

Chembiochem (2007): 933

An enzyme-linked immunosorbent assay for protein kinase D activity using phosphorylation site-specific antibodies

Authors:

Rykx A, Vancauwenbergh S, De Kimpe L, Janssens K, V and oninck S, Waelkens E, V and enheede JR, Van Lint J.

Journal:

Assay Drug Dev Technol (2007): 637

Implementation of high-content assay for inhibitors of mitogen-activated protein kinase phosphatases

Authors:

Vogt A, Lazo JS.

Journal:

Methods (2007): 268

High-throughput kinase assay based on surface plasmon resonance suitable for native protein substrates

Authors:

Takeda H, Fukumoto A, Miura A, Goshima N, Nomura N.

Journal:

Anal Biochem (2006): 262

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

Documents

Protocol
Safety Data Sheet (Catalog 31001)
Safety Data Sheet (Catalog 31002)
Certificate of Origin
Certificate of Analysis
Brochure: Cell Metabolism & Signaling Assays
Brochure: Enzyme Probes & Assay Kits

Telephone
Fax
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Page updated on October 29, 2025