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Annexin V-Biotin conjugate

<strong>Annexin V binding assay for identifying apoptosis cells. </strong> Jurkat cells were treated without (Green) or with 1uM staurosporine (Red)at 37 °C for 4-5 hours. Cells were then incubated with Annexin V-Biotin (Cat#20018), followed by incubation with streptavidin-iFluor® 488 (Cat#16955). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer FITC channel.
<strong>Annexin V binding assay for identifying apoptosis cells. </strong> Jurkat cells were treated without (Green) or with 1uM staurosporine (Red)at 37 °C for 4-5 hours. Cells were then incubated with Annexin V-Biotin (Cat#20018), followed by incubation with streptavidin-iFluor® 488 (Cat#16955). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer FITC channel.
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Catalog Number20018
Unit Size
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Telephone1-408-733-1055
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Physical properties
Molecular weight~36000
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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OverviewpdfSDSpdfProtocol


Molecular weight
~36000
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. There are a variety of parameters that can be used for monitoring cell viability. Annexin V conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). Annexin V-Biotin conjugate probe is one of the most of most sensitive Annexin V conjugates used for monitoring cell apoptosis in combination with our various fluorescent streptavidin conjugates.

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Annexin V-Cy3 conjugate55556915000010.1510.070.073
Annexin V-Cy5 conjugate65667025000010.2510.030.03
Annexin V-Cy7 conjugate7567792500000.30.050.036
Annexin V, XFD350 conjugate *XFD350 Same Structure to Alexa Fluor™ 350*34344119000-0.250.19
Annexin V, XFD488 conjugate *XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.30.11
Annexin V, XFD594 conjugate *XFD594 Same Structure to Alexa Fluor™ 594*590618920000.6610.430.56
Annexin V, PacBlue conjugate------