Annexin V-Biotin conjugate
Additional ordering information
|Shipping||Standard overnight for United States, inquire for international|
Storage, safety and handling
|H-phrase||H303, H313, H333|
|Intended use||Research Use Only (RUO)|
|R-phrase||R20, R21, R22|
|Storage||Freeze (< -15 °C); Minimize light exposure|
See also: Bioconjugation, Cell Cytotoxicity, Annexin-V Staining, Cellular Processes, Apoptosis and Necrosis, Biotin and Streptavidin
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. There are a variety of parameters that can be used for monitoring cell viability. Annexin V conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). Annexin V-Biotin conjugate probe is one of the most of most sensitive Annexin V conjugates used for monitoring cell apoptosis in combination with our various fluorescent streptavidin conjugates.
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Annexin V-Biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
|0.1 mg||0.5 mg||1 mg||5 mg||10 mg|
|1 mM||2.778 µL||13.889 µL||27.778 µL||138.889 µL||277.778 µL|
|5 mM||555.556 nL||2.778 µL||5.556 µL||27.778 µL||55.556 µL|
|10 mM||277.778 nL||1.389 µL||2.778 µL||13.889 µL||27.778 µL|
Enter any two values (mass, volume, concentration) to calculate the third.
|Mass (Calculate)||Molecular weight||Volume (Calculate)||Concentration (Calculate)||Moles|
|Annexin V, PacBlue conjugate|
Figure 1. Annexin V binding assay for identifying apoptosis cells. Jurkat cells were treated without (Green) or with 1uM staurosporine (Red)at 37 °C for 4-5 hours. Cells were then incubated with Annexin V-Biotin (Cat#20018), followed by incubation with streptavidin-iFluor® 488 (Cat#16955). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer FITC channel.