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Annexin V-Biotin conjugate

<strong>Annexin V binding assay for identifying apoptosis cells. </strong> Jurkat cells were treated without (Green) or with 1uM staurosporine (Red)at 37 °C for 4-5 hours. Cells were then incubated with Annexin V-Biotin (Cat#20018), followed by incubation with streptavidin-iFluor® 488 (Cat#16955). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer FITC channel.
<strong>Annexin V binding assay for identifying apoptosis cells. </strong> Jurkat cells were treated without (Green) or with 1uM staurosporine (Red)at 37 °C for 4-5 hours. Cells were then incubated with Annexin V-Biotin (Cat#20018), followed by incubation with streptavidin-iFluor® 488 (Cat#16955). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer FITC channel.
Ordering information
Price ()
Catalog Number20018
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight36000
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
36000
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. There are a variety of parameters that can be used for monitoring cell viability. Annexin V conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). Annexin V-Biotin conjugate probe is one of the most of most sensitive Annexin V conjugates used for monitoring cell apoptosis in combination with our various fluorescent streptavidin conjugates.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Annexin V-Biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM2.778 µL13.889 µL27.778 µL138.889 µL277.778 µL
5 mM555.556 nL2.778 µL5.556 µL27.778 µL55.556 µL
10 mM277.778 nL1.389 µL2.778 µL13.889 µL27.778 µL

Molarity calculator

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