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Annexin V-Cy5.5 conjugate
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). Cy5.5-Annexin V probe is one of the most popular fluorescent dye-Annexin V conjugates used for monitoring cell apoptosis.
Fluorescence images of HeLa cells labeled with Annexin V-Cy5.5 conjugate (Cat#20067). HeLa cells at 100,000 cells/well/100 µL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with (Right) or without (Left) 500 nM staurosporine (SS) at 37 ºC for 4 hours. Cells were then incubated with Annexin V-Cy5.5 conjugate (Red) and measured using fluorescence microscope with a Cy5 filter. Viable cells were labeled with Cellbrite™ Orange (Shown as Green).
Fluorescence images of HeLa cells labeled with Annexin V-Cy5.5 conjugate (Cat#20067). HeLa cells at 100,000 cells/well/100 µL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with (Right) or without (Left) 500 nM staurosporine (SS) at 37 ºC for 4 hours. Cells were then incubated with Annexin V-Cy5.5 conjugate (Red) and measured using fluorescence microscope with a Cy5 filter. Viable cells were labeled with Cellbrite™ Orange (Shown as Green).
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
20067100 Tests
Price
 
Physical properties
Molecular weight~36000
SolventWater
Spectral properties
Correction factor (260 nm)0.097
Correction factor (280 nm)0.094
Extinction coefficient (cm -1 M -1)
220000
1
Excitation (nm)684
Emission (nm)701
Quantum yield
0.23
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation633 or 640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Flyer: Annexin V Conjugates
Flyer: Flow Cytometry Fluorochromes
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Page updated on October 8, 2024