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Annexin V-Cy5 conjugate
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). Cy5-Annexin V probe is one of the most popular fluorescent dye-Annexin V conjugates used for monitoring cell apoptosis.
The binding activity of Annexin V-Cy5 conjugate to phosphatidylserine (PS) residues in Jurkat cells. Jurkat cells were treated without (Blue) or with 1 μM staurosporine (Red) for 4-5 hours in a 37°C, 5% CO2 incubator, and then dye-loaded for 30 minutes. The fluorescence intensity of Annexin V-Cy5 was measured with a FACSCalibur (Becton Dickinson, San Jose, CA) flow cytometer using the FL4 channel.
The binding activity of Annexin V-Cy5 conjugate to phosphatidylserine (PS) residues in Jurkat cells. Jurkat cells were treated without (Blue) or with 1 μM staurosporine (Red) for 4-5 hours in a 37°C, 5% CO2 incubator, and then dye-loaded for 30 minutes. The fluorescence intensity of Annexin V-Cy5 was measured with a FACSCalibur (Becton Dickinson, San Jose, CA) flow cytometer using the FL4 channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
20066100 Tests
Price
 
Physical properties
Molecular weight~36000
SolventWater
Spectral properties
Correction factor (260 nm)0.03
Correction factor (280 nm)0.03
Correction factor (656 nm)0.0793
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)656
Emission (nm)670
Quantum yield
0.25
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 8, 2024