Annexin V, FITC Labeled

The detection of binding activity of FITC-Annexin V conjugate to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.

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Physical properties

Molecular weight	~36000
Solvent	Water

Spectral properties

Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

~36000

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

73000

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.92

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent FITC-Annexin V conjugate is widely used for monitoring cell apoptosis with a flow cytometer or a fluorescence microscope. For microplate assays of annexin V-based cell apoptosis detection, we recommend you use our Cell Meter™ kits.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
Annexin V, TRITC Labeled	544	570	100000	0.27	0.34

Images

Figure 1. The detection of binding activity of FITC-Annexin V conjugate to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with FITC-Annexin V conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.

Citations

View all 1 citations: Citation Explorer

Response of Mammalian cells to non-thermal intense narrowband pulsed electric fields
Authors: Katsuki, Sunao and Li, Yulan and Miyakawa, Daiki and Yamada, Ryo and Onishi, Nobuaki and Lim, Soowon
Journal: (2017): 1358--1361

References

View all 90 references: Citation Explorer

Annexin A5-functionalized bimodal lipid-based contrast agents for the detection of apoptosis
Authors: van Tilborg GA, Mulder WJ, Deckers N, Storm G, Reutelingsperger CP, Strijkers GJ, Nicolay K.
Journal: Bioconjug Chem (2006): 741

Annexin V/beta5 integrin interactions regulate apoptosis of growth plate chondrocytes
Authors: Wang W, Kirsch T.
Journal: J Biol Chem (2006): 30848

Myocyte apoptosis during acute myocardial infarction in rats is related to early sarcolemmal translocation of annexin A5 in border zone
Authors: Monceau V, Belikova Y, Kratassiouk G, Robidel E, Russo-Marie F, Charlemagne D.
Journal: Am J Physiol Heart Circ Physiol (2006): H965

Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924

In-vivo visualization of radiation-induced apoptosis using (125)I-annexin V
Authors: Watanabe H, Murata Y, Miura M, Hasegawa M, Kawamoto T, Shibuya H.
Journal: Nucl Med Commun (2006): 81

Mapping of treatment-induced apoptosis in normal structures: 99mTc-Hynic-rh-annexin V SPECT and CT image fusion
Authors: Kartachova MS, Valdes Olmos RA, Haas RL, Hoebers FJ, van den Brekel MW, van Z and wijk N, Herk M, Verheij M.
Journal: Eur J Nucl Med Mol Imaging (2006): 893

Non-fusion expression in Escherichia coli: Single-step purification of recombinant human annexin A5 for detection of apoptosis
Authors: Wang F, He XW, Yan HL, Huang JJ, Zhang Y, Jiang L, Gao YJ, Sun SH.
Journal: Protein Expr Purif (2006): 80

Quantum dots based probes conjugated to annexin V for photostable apoptosis detection and imaging
Authors: Le Gac S, Vermes I, van den Berg A.
Journal: Nano Lett (2006): 1863

Lymphoma cells protected from apoptosis by dysregulated bcl-2 continue to bind annexin V in response to B-cell receptor engagement: a cautionary tale
Authors: Holder MJ, Barnes NM, Gregory CD, Gordon J.
Journal: Leuk Res (2006): 77

The impact of altered annexin I protein levels on apoptosis and signal transduction pathways in prostate cancer cells
Authors: Hsiang CH, Tunoda T, Whang YE, Tyson DR, Ornstein DK.
Journal: Prostate (2006): 1413