Annexin V | AAT Bioquest

Annexin V, FITC Labeled

FITC Annexin V is a highly fluorescent conjugate used to identify and quantitate apoptotic cells by flow cytometry.

High Sensitivity and Specificity to efficiently detect early apoptotic cells by binding to exposed PS.
Dual Staining Capability can be used in conjunction with propidium iodide (PI) to distinguish between apoptotic and necrotic cells.
Non-Radioactive Assay provides a safe and easy method for apoptosis detection without the need for radioactive materials.
Rapid and Simple Protocol enables minimal hands-on time with straightforward staining and analysis procedures.
Versatile Applications suitable for use in various cell types and experimental conditions.

Binding activity of FITC-Annexin V conjugate to phosphatidylserine (PS) residues in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) at 37°C for 4 hours and then labeled with FITC-Annexin V conjugate for 30 minutes. The fluorescence intensity was measured using an ACEA NovoCyte flow cytometer in the FITC channel.

Catalog	Size	Price	Quantity
20030	100 tests	Price
20032	300 tests	Price

Overview

Annexin V is a 35-36 kDa phospholipid-binding protein that has a high affinity for phosphatidylserine (PS) residues. In apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, making it accessible to Annexin V conjugated to fluorescein isothiocyanate (FITC). By binding to PS, Annexin V FITC can be used to detect and quantify apoptotic cells via flow cytometry or fluorescence microscopy.

Mechanism of Action

During apoptosis, the integrity of the plasma membrane changes, leading to the translocation of phosphatidylserine (PS) from the inner to the outer leaflet. Annexin V FITC, a fluorophore-conjugated protein with a high affinity for PS, selectively binds to the externalized PS residues. This binding event is detectable and quantifiable via flow cytometry or fluorescence microscopy. The intensity of the FITC signal enables precise identification and quantification of apoptotic cells, as it directly correlates with Annexin V binding to PS on the cell surface.

Citations

View all 3 citations: Citation Explorer

Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer

Authors:

Zhang, Lei and Chen, Wenbang and Li, Xiaojun and Wang, Gengming and Xing, Fubao and Zhu, Xiao

Journal:

Cell Adhesion \& Migration (2024): 1--11

The role of non-coding RNA ASBEL regulation of BTG3 in pancreatic ductal adenocarcinoma tumor progression

Authors:

Chen, Jing and Zhu, Ming-Yuan and Huang, Yan-Hua and Ling, Yi-Ting and Gu, Tian-Yuan and Zhou, Quan and Fei, Ming-Jian and Zhou, Zhong-Cheng

Journal:

Current Therapeutic Research (2023): 100700

Response of Mammalian cells to non-thermal intense narrowband pulsed electric fields

Authors:

Katsuki, Sunao and Li, Yulan and Miyakawa, Daiki and Yamada, Ryo and Onishi, Nobuaki and Lim, Soowon

References

View all 90 references: Citation Explorer

Non-fusion expression in Escherichia coli: Single-step purification of recombinant human annexin A5 for detection of apoptosis

Authors:

Wang F, He XW, Yan HL, Huang JJ, Zhang Y, Jiang L, Gao YJ, Sun SH.

Journal:

Protein Expr Purif (2006): 80

PET imaging of apoptosis with (64)Cu-labeled streptavidin following pretargeting of phosphatidylserine with biotinylated annexin-V

Authors:

Cauchon N, Langlois R, Rousseau JA, Tessier G, Cadorette J, Lecomte R, Hunting DJ, Pavan RA, Zeisler SK, van Lier JE.

Journal:

Eur J Nucl Med Mol Imaging. (2006)

The impact of altered annexin I protein levels on apoptosis and signal transduction pathways in prostate cancer cells

Authors:

Hsiang CH, Tunoda T, Whang YE, Tyson DR, Ornstein DK.

Journal:

Prostate (2006): 1413

Lymphoma cells protected from apoptosis by dysregulated bcl-2 continue to bind annexin V in response to B-cell receptor engagement: a cautionary tale

Authors:

Holder MJ, Barnes NM, Gregory CD, Gordon J.

Journal:

Leuk Res (2006): 77

Quantum dots based probes conjugated to annexin V for photostable apoptosis detection and imaging

Authors:

Le Gac S, Vermes I, van den Berg A.

Journal:

Nano Lett (2006): 1863

Physical properties

Molecular weight	~36000
Solvent	Water

Spectral properties

Absorbance (nm)	488
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 8, 2024