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Annexin V, FITC Labeled
FITC Annexin V is a highly fluorescent conjugate used to identify and quantitate apoptotic cells by flow cytometry.

Product Description

Annexin V is a 35-36 kDa phospholipid-binding protein that has a high affinity for phosphatidylserine (PS) residues. In apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, making it accessible to Annexin V conjugated to fluorescein isothiocyanate (FITC). By binding to PS, Annexin V FITC can be used to detect and quantify apoptotic cells via flow cytometry or fluorescence microscopy.

Key Features

  • High Sensitivity and Specificity to efficiently detect early apoptotic cells by binding to exposed PS.
  • Dual Staining Capability can be used in conjunction with propidium iodide (PI) to distinguish between apoptotic and necrotic cells.
  • Non-Radioactive Assay provides a safe and easy method for apoptosis detection without the need for radioactive materials.
  • Rapid and Simple Protocol enables minimal hands-on time with straightforward staining and analysis procedures.
  • Versatile Applications suitable for use in various cell types and experimental conditions.

Mechanism of Action

During apoptosis, the integrity of the plasma membrane changes, leading to the translocation of phosphatidylserine (PS) from the inner to the outer leaflet. Annexin V FITC, a fluorophore-conjugated protein with a high affinity for PS, selectively binds to the externalized PS residues. This binding event is detectable and quantifiable via flow cytometry or fluorescence microscopy. The intensity of the FITC signal enables precise identification and quantification of apoptotic cells, as it directly correlates with Annexin V binding to PS on the cell surface.
Binding activity of FITC-Annexin V conjugate to phosphatidylserine (PS) residues in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) at 37°C for 4 hours and then labeled with FITC-Annexin V conjugate for 30 minutes. The fluorescence intensity was measured using an ACEA NovoCyte flow cytometer in the FITC channel.
Binding activity of FITC-Annexin V conjugate to phosphatidylserine (PS) residues in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) at 37°C for 4 hours and then labeled with FITC-Annexin V conjugate for 30 minutes. The fluorescence intensity was measured using an ACEA NovoCyte flow cytometer in the FITC channel.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
20030100 tests
Price
20032300 tests
Price
 
Physical properties
Molecular weight~36000
SolventWater
Spectral properties
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)491
Emission (nm)516
Quantum yield
0.92
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Flyer: Annexin V Conjugates
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Page updated on October 8, 2024