Annexin V | AAT Bioquest

Annexin V-iFluor® 488 conjugate

Annexin V-iFluor® 488 Conjugate is a green fluorescent probe for detecting phosphatidylserine exposure on apoptotic cells using flow cytometry and fluorescence microscopy.

Fluorescence Detection: Excitation at 491 nm and emission at 516 nm.
Apoptosis Detection: Detects phosphatidylserine exposure during early-stage apoptosis.
Calcium-Dependent Binding: Annexin V selectively binds phosphatidylserine in the presence of calcium.
Instrument Compatibility: Supports 488 nm laser and FITC filter set.
Multiplex Compatibility: Compatible with propidium iodide for distinguishing apoptotic and dead cells.

The detection of binding activity of Annexin V-iFluor® 488 and phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Blue) or with 20 µM camptothecin (Red) in a 37 °C, 5% CO2 incubator for 4-5 hours, and then dye loaded with Annexin V-iFluor® 488 for 30 minutes. The fluorescence intensity of Annexin V-iFluor® 488 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using the FL1 channel.

Protocol

SDS

Catalog	Size	Price	Quantity
20071	100 tests	Price

Overview

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent Annexin V conjugate has spectral properties similar to Alexa Fluor® 488 (Alexa Fluor® 488 is the trademark of Invitrogen) and FITC.

Citations

View all 11 citations: Citation Explorer

TRIM28-Derived Peptide Exerts Anti-Tumor Roles by Stabilizing Tumor Suppressive BRD7 Protein in Multiple Cancers

Authors:

Wei, Qingqing and Xue, Changning and Li, Mengna and Wei, Jianxia and Zheng, Lemei and Chen, Shipeng and Wang, Yijie and Duan, Yumei and Luo, Yanwei and Xiong, Wei and others,

Journal:

Advanced Science (2026): e76974

Polymersomal delivery enhances third-generation photodynamic therapy with the first white-light-activated peptide photosensitiser for melanoma

Authors:

Khan, Talat Nahid and Khan, Mohammed Naeem and Oduor, Poullette R and Nesbitt, Heather and McKaig, Thomas and McHale, Anthony P and Callan, John F and Callan, Bridgeen

Journal:

RSC Pharmaceutics (2026)

Synthesis and Biological Activity of 2-Chloro-8-methoxy-5-methyl-5 H-indolo [2, 3-b] Quinoline for the Treatment of Colorectal Cancer by Modulating PI3K/AKT/mTOR Pathways

Authors:

Ma, Yunhao and Zhu, Hongmei and Jiang, Xinrong and Zhou, Zhongkun and Zhou, Yong and Tian, Yanan and Tu, Lixue and Lu, Juan and Niu, Yuqing and Du, Liqian and others,

Journal:

ACS Omega (2024)

Deoxynivalenol induces m6A-mediated upregulation of p21 and growth arrest of mouse hippocampal neuron cells in vitro

Authors:

Xu, Peirong and Zhao, Yulan and Feng, Yue and Zhao, Mindie and Zhao, Ruqian

Journal:

Cell Biology and Toxicology (2024): 1--15

A novel selective estrogen receptor degrader induces cell cycle arrest in breast cancer via ER$\alpha$ degradation and the autophagy-lysosome pathway

Authors:

Zhou, Jiawei and Shen, Rong and Liu, Jing and Deng, Xiangping and Xin, Lilan and Zhou, Hai-Bing and Huang, Jian

Journal:

Bioorganic \& Medicinal Chemistry (2023): 117235

References

View all 32 references: Citation Explorer

Gold fluorescent annexin A5 as a novel apoptosis detection tool

Authors:

Kurschus FC, Pal PP, Baumler P, Jenne DE, Wiltschi B, Budisa N.

Journal:

Cytometry A (2009): 626

Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis

Authors:

Wang CY, Lin YS, Su WC, Chen CL, Lin CF.

Journal:

Mol Biol Cell (2009): 4153

Evaluation of annexin V and Calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection

Authors:

Palma PF, Baggio GL, Spada C, Silva RD, Ferreira SI, Treitinger A.

Journal:

Braz J Infect Dis (2008): 108

Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

Authors:

Chen S, Cheng AC, Wang MS, Peng X.

Journal:

World J Gastroenterol (2008): 2174

Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells

Authors:

Hu T, Shi J, Jiao X, Zhou J, Yin X.

Journal:

Braz J Med Biol Res (2008): 750

Physical properties

Molecular weight	~36000
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 2, 2026