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Annexin V-iFluor® 555 conjugate
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent Annexin V conjugate has spectral properties similar to Alexa Fluor® 555 (Alexa Fluor® 555 is the trademark of Invitrogen) and Cy3® (Cy3® is the trademark of GE Healthcare).
Jurkat cells were treated with 1 µM staurosporine for 4 hours to induce apoptosis. Following treatment, cells were stained with Annexin V-iFluor® 555 conjugate (Cat No. 20072). Nuclei were labeled with Nuclear Green™ DCS1 (Cat No. 17550). Images were acquired on a confocal microscope.
Jurkat cells were treated with 1 µM staurosporine for 4 hours to induce apoptosis. Following treatment, cells were stained with Annexin V-iFluor® 555 conjugate (Cat No. 20072). Nuclei were labeled with Nuclear Green™ DCS1 (Cat No. 17550). Images were acquired on a confocal microscope.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
20072100 tests
Price
 
Physical properties
Molecular weight~36000
SolventWater
Spectral properties
Correction factor (260 nm)0.23
Correction factor (280 nm)0.14
Extinction coefficient (cm -1 M -1)
100000
1
Excitation (nm)557
Emission (nm)570
Quantum yield
0.64
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 or 532 nm laser
Emission575/26 nm filter
Instrument specification(s)PE channel
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 8, 2024