Annexin V | AAT Bioquest

Annexin V-iFluor® 594 conjugate

Annexin V-iFluor® 594 Conjugate is a red fluorescent probe for detecting phosphatidylserine exposure on apoptotic cells using flow cytometry and fluorescence microscopy.

Fluorescence Detection: Excitation at 587 nm and emission at 603 nm.
Apoptosis Detection: Detects phosphatidylserine exposure during early-stage apoptosis.
Calcium-Dependent Binding: Annexin V binds exposed phosphatidylserine in the presence of calcium.
Instrument Compatibility: Supports 532/561 nm laser and Cy3/TRITC filter set.
Multiplex Compatibility: Compatible with dead-cell stains for distinguishing apoptotic and membrane-compromised cells.

Images of Jurkat cells in a Costar black wall/clear bottom 96-well plate stained with Annexin V-iFluor® 594 conjugate. (Left): Untreated control cells. (Right): Cells treated with 20 µM camptothecin for 5 hours.

Protocol

SDS

Catalog	Size	Price	Quantity
20073	100 tests	Price

Overview

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent Annexin V conjugate has spectral properties similar to Alexa Fluor® 594 and Texas Red®(Texas Red® and Alexa Fluor® 594 are the trademarks of Invitrogen).

Citations

View all 5 citations: Citation Explorer

VEGFR2 as a target for CAR T cell therapy of Ewing sarcoma

Authors:

Englisch, Alexander and Altvater, Bianca and Kailayangiri, Sareetha and Hartmann, Wolfgang and Rossig, Claudia

Journal:

Pediatric Blood \& Cancer (2020): e28313

Brain HIV-1 latently-infected reservoirs targeted by the suicide gene strategy

Authors:

Saeb, Sepideh and Ravanshad, Mehrdad and Pourkarim, Mahmoud Reza and Daouad, Fadoua and Baesi, Kazem and Rohr, Olivier and Wallet, Cl{\'e}mentine and Schwartz, Christian

Encapsulation of Apoptotic Proteins in Lipid Nanoparticles to Induce Death of Cancer Cells

Authors:

Bae, Chun-Sik and Lee, Chang-Min and Ahn, Taeho

Journal:

Biotechnology and Bioprocess Engineering (2020): 1--8

Resources-Application Notes Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy

Authors:

Larson, Brad and Banks, Peter

Journal:

Experimental Biology (2017)

Targeted Magnetic Intra-Lysosomal Hyperthermia produces lysosomal reactive oxygen species and causes Caspase-1 dependent cell death

Authors:

Clerc, Pascal and Jeanjean, Pauline and Halalli, Nicolas and Gougeon, Michel and Pipy, Bernard and Carrey, Julian and Fourmy, Daniel and Gigoux, Véronique

Journal:

Journal of Controlled Release (2017)

References

View all 32 references: Citation Explorer

Gold fluorescent annexin A5 as a novel apoptosis detection tool

Authors:

Kurschus FC, Pal PP, Baumler P, Jenne DE, Wiltschi B, Budisa N.

Journal:

Cytometry A (2009): 626

Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis

Authors:

Wang CY, Lin YS, Su WC, Chen CL, Lin CF.

Journal:

Mol Biol Cell (2009): 4153

Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

Authors:

Chen S, Cheng AC, Wang MS, Peng X.

Journal:

World J Gastroenterol (2008): 2174

Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells

Authors:

Hu T, Shi J, Jiao X, Zhou J, Yin X.

Journal:

Braz J Med Biol Res (2008): 750

Evaluation of annexin V and Calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection

Authors:

Palma PF, Baggio GL, Spada C, Silva RD, Ferreira SI, Treitinger A.

Journal:

Braz J Infect Dis (2008): 108

Physical properties

Molecular weight	~36000
Solvent	Water

Spectral properties

Absorbance (nm)	587
Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	532 or 561 nm laser
Emission	610/20 nm filter
Instrument specification(s)	PE-Texas Red channel

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 2, 2026