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Annexin V-iFluor® 594 conjugate
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent Annexin V conjugate has spectral properties similar to Alexa Fluor® 594 and Texas Red®(Texas Red® and Alexa Fluor® 594 are the trademarks of Invitrogen).
Images of Jurkat cells in a Costar black wall/clear bottom 96-well plate stained with Annexin V-iFluor® 594 conjugate. (Left): Untreated control cells. (Right): Cells treated with 20 µM camptothecin for 5 hours.
Images of Jurkat cells in a Costar black wall/clear bottom 96-well plate stained with Annexin V-iFluor® 594 conjugate. (Left): Untreated control cells. (Right): Cells treated with 20 µM camptothecin for 5 hours.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
20073100 tests
Price
 
Physical properties
Molecular weight~36000
SolventWater
Spectral properties
Absorbance (nm)587
Correction factor (260 nm)0.05
Correction factor (280 nm)0.04
Extinction coefficient (cm -1 M -1)
200000
1
Excitation (nm)587
Emission (nm)603
Quantum yield
0.53
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation532 or 561 nm laser
Emission610/20 nm filter
Instrument specification(s)PE-Texas Red channel
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2024