Annexin V | AAT Bioquest

Annexin V-iFluor® 633 conjugate

Annexin V-iFluor® 633 Conjugate is a far-red fluorescent probe for detecting phosphatidylserine exposure on apoptotic cells using flow cytometry and fluorescence microscopy.

Fluorescence Detection: Excitation at 640 nm and emission at 654 nm.
Apoptosis Detection: Detects phosphatidylserine exposure during early-stage apoptosis.
Calcium-Dependent Binding: Annexin V binds exposed phosphatidylserine in the presence of calcium.
Instrument Compatibility: Supports 633/640 nm laser excitation and Cy5 microscopy filters.
Multiplex Compatibility: Compatible with dead-cell stains for distinguishing apoptotic and membrane-compromised cells.

The detection of binding activity of Annexin V-iFluor® 633 conjugate to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with Annexin V-iFluor® 633 conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in APC channel.

Protocol

SDS

Catalog	Size	Price	Quantity
20069	100 tests	Price

Overview

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. iFluor® 633-Annexin V conjugate has spectral properties similar to Alexa Fluor® 633 with fluorescence excitation and emission maxima of ~638 nm and ~655 nm respectively (Alexa Fluor® is the trademark of Invitrogen).

Citations

View all 4 citations: Citation Explorer

A drug-like imidazole-benzothiazole conjugate inhibits malignant melanoma by stabilizing the c-MYC G-quadruplex

Authors:

Wu, Tian-Ying and Huang, Qiong and Huang, Zhi-Shu and Hu, Ming-Hao and Tan, Jia-Heng

Journal:

Bioorganic Chemistry (2020): 103866

New substituted quinoxalines inhibit triple-negative breast cancer by specifically downregulating the c-MYC transcription

Authors:

Hu, Ming-Hao and Wu, Tian-Ying and Huang, Qiong and Jin, Guangyi

Journal:

Nucleic acids research (2019)

Resources-Application Notes Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy

Authors:

Larson, Brad and Banks, Peter

Journal:

Experimental Biology (2017)

Targeted Magnetic Intra-Lysosomal Hyperthermia produces lysosomal reactive oxygen species and causes Caspase-1 dependent cell death

Authors:

Clerc, Pascal and Jeanjean, Pauline and Halalli, Nicolas and Gougeon, Michel and Pipy, Bernard and Carrey, Julian and Fourmy, Daniel and Gigoux, Véronique

Journal:

Journal of Controlled Release (2017)

References

View all 32 references: Citation Explorer

Gold fluorescent annexin A5 as a novel apoptosis detection tool

Authors:

Kurschus FC, Pal PP, Baumler P, Jenne DE, Wiltschi B, Budisa N.

Journal:

Cytometry A (2009): 626

Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis

Authors:

Wang CY, Lin YS, Su WC, Chen CL, Lin CF.

Journal:

Mol Biol Cell (2009): 4153

Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells

Authors:

Hu T, Shi J, Jiao X, Zhou J, Yin X.

Journal:

Braz J Med Biol Res (2008): 750

Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

Authors:

Chen S, Cheng AC, Wang MS, Peng X.

Journal:

World J Gastroenterol (2008): 2174

Evaluation of annexin V and Calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection

Authors:

Palma PF, Baggio GL, Spada C, Silva RD, Ferreira SI, Treitinger A.

Journal:

Braz J Infect Dis (2008): 108

Physical properties

Molecular weight	~36000
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.062
Correction factor (280 nm)	0.044
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	640
Emission (nm)	654
Quantum yield	0.29 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	633 or 640 nm laser
Emission	660/20 nm filter
Instrument specification(s)	APC channel

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 2, 2026