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Annexin V-iFluor® 633 conjugate
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. iFluor 633-Annexin V conjugate has spectral properties similar to Alexa Fluor® 633 with fluorescence excitation and emission maxima of ~638 nm and ~655 nm respectively (Alexa Fluor® is the trademark of Invitrogen).
The detection of binding activity of Annexin V-iFluor® 633 conjugate to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with Annexin V-iFluor® 633 conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in APC channel.
The detection of binding activity of Annexin V-iFluor® 633 conjugate to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) in 37 ºC for 4 hours, and then labeled with Annexin V-iFluor® 633 conjugate for 30 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in APC channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
20069100 tests
Price
 
Physical properties
Molecular weight~36000
SolventWater
Spectral properties
Correction factor (260 nm)0.062
Correction factor (280 nm)0.044
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)640
Emission (nm)654
Quantum yield
0.29
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation633 or 640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2024