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Annexin V-iFluor® 647 conjugate
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent Annexin V conjugate has spectral properties similar to Alexa Fluor® 647 (Alexa Fluor® 647 is the trademark of Invitrogen) and Cy5® (Cy5® is the trademark of GE Healthcare).
The detection of binding activity of Annexin V-iFluor® 647 and phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Blue) or with 20 µM staurosporine(Red) in a 37 °C, 5% CO2 incubator for 4-5 hours, and then dye loaded with Annexin V-iFluor® 647 for 30 minutes. The fluorescence intensity of Annexin V-iFluor® 647 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using the FL4 channel.
The detection of binding activity of Annexin V-iFluor® 647 and phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Blue) or with 20 µM staurosporine(Red) in a 37 °C, 5% CO2 incubator for 4-5 hours, and then dye loaded with Annexin V-iFluor® 647 for 30 minutes. The fluorescence intensity of Annexin V-iFluor® 647 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using the FL4 channel.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
20074100 tests
Price
 
Physical properties
Molecular weight~36000
SolventWater
Spectral properties
Correction factor (260 nm)0.03
Correction factor (280 nm)0.03
Correction factor (656 nm)0.0793
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)656
Emission (nm)670
Quantum yield
0.25
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Flyer: Annexin V Conjugates
Flyer: Flow Cytometry Fluorochromes
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Page updated on October 8, 2024