Annexin V | AAT Bioquest

Annexin V-iFluor® 750 conjugate

Annexin V-iFluor® 750 Conjugate is a near-infrared fluorescent probe for detecting phosphatidylserine exposure on apoptotic cells using flow cytometry and fluorescence microscopy.

Fluorescence Detection: Excitation at 757 nm and emission at 779 nm.
Apoptosis Detection: Detects phosphatidylserine exposure during early-stage apoptosis.
Calcium-Dependent Binding: Annexin V binds exposed phosphatidylserine in the presence of calcium.
Instrument Compatibility: Supports 633/640 nm laser and Cy7 filter set.
Multiplex Compatibility: Compatible with dead-cell stains for distinguishing apoptotic and membrane-compromised cells.

The detection of binding activity of Annexin V-iFluor® 750 conjugate to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) at 37 ºC for 4 hours and then labeled with Annexin V-iFluor® 750 conjugate for 30 minutes. Fluorescence intensity was measured using an ACEA NovoCyte flow cytometer in the APC-Cy7 channel.

Protocol

SDS

Catalog	Size	Price	Quantity
20076	100 tests	Price

Overview

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent Annexin V conjugate has spectral properties similar to Alexa Fluor® 750 (Alexa Fluor® 750 is the trademark of Invitrogen) and Cy7® (Cy7® is the trademark of GE Healthcare).

Citations

View all 2 citations: Citation Explorer

Resources-Application Notes Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy

Authors:

Larson, Brad and Banks, Peter

Journal:

Experimental Biology (2017)

Targeted Magnetic Intra-Lysosomal Hyperthermia produces lysosomal reactive oxygen species and causes Caspase-1 dependent cell death

Authors:

Clerc, Pascal and Jeanjean, Pauline and Halalli, Nicolas and Gougeon, Michel and Pipy, Bernard and Carrey, Julian and Fourmy, Daniel and Gigoux, Véronique

Journal:

Journal of Controlled Release (2017)

References

View all 32 references: Citation Explorer

Gold fluorescent annexin A5 as a novel apoptosis detection tool

Authors:

Kurschus FC, Pal PP, Baumler P, Jenne DE, Wiltschi B, Budisa N.

Journal:

Cytometry A (2009): 626

Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis

Authors:

Wang CY, Lin YS, Su WC, Chen CL, Lin CF.

Journal:

Mol Biol Cell (2009): 4153

Evaluation of annexin V and Calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection

Authors:

Palma PF, Baggio GL, Spada C, Silva RD, Ferreira SI, Treitinger A.

Journal:

Braz J Infect Dis (2008): 108

Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

Authors:

Chen S, Cheng AC, Wang MS, Peng X.

Journal:

World J Gastroenterol (2008): 2174

Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells

Authors:

Hu T, Shi J, Jiao X, Zhou J, Yin X.

Journal:

Braz J Med Biol Res (2008): 750

Physical properties

Molecular weight	~36000
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.044
Correction factor (280 nm)	0.039
Correction factor (565 nm)	0.0250
Correction factor (650 nm)	0.1413
Extinction coefficient (cm ^-1 M ^-1)	275000 ¹
Excitation (nm)	757
Emission (nm)	779
Quantum yield	0.12 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	633 or 640 nm laser
Emission	780LP filter
Instrument specification(s)	APC-Cy7 channel

Fluorescence microscope
Excitation	Cy7 filter set
Emission	Cy7 filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 2, 2026