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Annexin V-mFluor™ Blue 570 conjugate
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. The green fluorescent stain is well excited with the Blue Laser at 488 nm, and emits intense orange fluorescence at ~570 nm. Its emission is much stronger than those of Annexin V-Cy3 with 488 nm excitation.
The detection of binding activity of Annexin V-mFluor™ Blue 570 to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) at 37 °C for 4 hours and then labeled with Annexin V-mFluor™ Blue 570 conjugate for 30 minutes.
The detection of binding activity of Annexin V-mFluor™ Blue 570 to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) at 37 °C for 4 hours and then labeled with Annexin V-mFluor™ Blue 570 conjugate for 30 minutes.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
20085100 tests
Price
 
Physical properties
Molecular weight~36000
SolventWater
Spectral properties
Absorbance (nm)553
Correction factor (260 nm)0.228
Correction factor (280 nm)0.179
Extinction coefficient (cm -1 M -1)
120000
1
Excitation (nm)505
Emission (nm)564
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 or 532 nm laser
Emission575/26 nm filter
Instrument specification(s)PE channel
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2024