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Annexin V-mFluor™ Violet 540 conjugate
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent Annexin V conjugate has spectral properties similar to Pacific Orange® (Pacific Orange® is the trademark of Invitrogen). The orange fluorescent stain is well excited with the Violet Laser at 405 nm, and emits intense blue fluorescence at ~560 nm.
The detection of binding activity of Annexin V-mFluor™ Violet 540 conjugate to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) at 37 °C for 4 hours and then labeled with Annexin V-mFluor™ Violet 540 conjugate for 30 minutes. Fluorescence intensity was measured using an ACEA NovoCyte flow cytometer in the Pacific Orange channel.
The detection of binding activity of Annexin V-mFluor™ Violet 540 conjugate to phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Green) or with 1 μM staurosporine (Red) at 37 °C for 4 hours and then labeled with Annexin V-mFluor™ Violet 540 conjugate for 30 minutes. Fluorescence intensity was measured using an ACEA NovoCyte flow cytometer in the Pacific Orange channel.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
20082100 tests
Price
 
Physical properties
Molecular weight~36000
SolventWater
Spectral properties
Absorbance (nm)401
Correction factor (260 nm)1.326
Correction factor (280 nm)0.543
Extinction coefficient (cm -1 M -1)
18000
1
Excitation (nm)402
Emission (nm)535
Quantum yield
0.21
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation405 nm laser
Emission530/30 nm filter
Instrument specification(s)Pacific Orange channel
Fluorescence microscope
ExcitationDAPI filter
EmissionTRITC filter
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Flyer: Annexin V Conjugates
Flyer: Flow Cytometry Fluorochromes
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Page updated on October 8, 2024