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Annexin V, TRITC Labeled
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent TRITC-Annexin V conjugate is widely used for monitoring cell apoptosis with a flow cytometer or a fluorescence microscope. For microplate assays of annexin V-based cell apoptosis detection, we recommend you use our Cell Meter™ kits.
<p>Fluorescence image of Jurkat cells stained with TRITC-Annexin V. Jurkat cells were treated without (Left) or with 1 &mu;M staurosporine (Right) in 37 &ordm;C for 4 hours. The fluorescence intensity was measured using a fluorescence microscope with TRITC filter set.</p>
<p>Fluorescence image of Jurkat cells stained with TRITC-Annexin V. Jurkat cells were treated without (Left) or with 1 &mu;M staurosporine (Right) in 37 &ordm;C for 4 hours. The fluorescence intensity was measured using a fluorescence microscope with TRITC filter set.</p>
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
20031100 tests
Price
 
Physical properties
Molecular weight~36000
SolventWater
Spectral properties
Correction factor (260 nm)0.27
Correction factor (280 nm)0.34
Extinction coefficient (cm -1 M -1)
100000
Excitation (nm)544
Emission (nm)570
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 or 532 nm laser
Emission575/26 nm filter
Instrument specification(s)PE channel
Fluorescence microscope
ExcitationTRITC filter set
EmissionTRITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Flyer: Annexin V Conjugates
Flyer: Flow Cytometry Fluorochromes
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Page updated on October 8, 2024