APC-Cy7 Tandem
Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. APC-Cy7 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~780 nm. AAT Bioquest also offers a unique preactivated APC-Cy7 to facilitate the APC-Cy7 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy7 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy7 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
![Flow cytometry analysis of whole blood stained with APC-Cy7 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-Cy7 specific R7-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fapc-cy7-tandem%2Ffigure-for-apc-cy7-tandem_1PCVw.png&w=640&q=75)
![Flow cytometry analysis of whole blood stained with APC-Cy7 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-Cy7 specific R7-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fapc-cy7-tandem%2Ffigure-for-apc-cy7-tandem_1PCVw.png&w=640&q=75)
![Flow cytometry analysis of whole blood stained with APC-Cy7 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-Cy7 specific R7-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fapc-cy7-tandem%2Ffigure-for-apc-cy7-tandem_1PCVw.png&w=128&q=25)
![Flow cytometry analysis of PBMC stained with APC-Cy7 anti-human CD3 *UCHT1* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-Cy7 specific R7-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fapc-cy7-tandem%2Ffigure-for-apc-cy7-tandem_xUeYK.png&w=128&q=25)
![Flow cytometry analysis of whole blood stained with APC-Cy7 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-Cy7 specific R7-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fapc-cy7-tandem%2Ffigure-for-apc-cy7-tandem_WHyJf.png&w=128&q=25)
![Human peripheral blood lymphocytes were stained with APC-Cy7 anti-Human CD4 (clone SK3, mouse IgG1, κ) conjugate prepared with APC-Cy7 Tandem (Cat# 2625). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer APC-Cy7 Channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fapc-cy7-tandem%2Ffigure-for-apc-cy7-tandem_NSRwy.jpg&w=128&q=25)
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
PE-Cy7 Tandem | 565 | 778 | 1960000 |
APC-XFD700 Tandem | 651 | 707 | 700000 |
APC-XFD750 Tandem | 651 | 776 | 700000 |
References
View all 46 references: Citation Explorer
Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573
Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016
Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598
Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113
Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59