APC-iFluor® 700 Tandem
Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. Its strong absorption at 633 nm and 647 nm makes the tandem one of the best fluorophores used with a red laser in flow cytometry applications. APC-XFD 700 has the same structure to Alexa Fluor® 700 tandem. It is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at ~710 nm.
![Flow cytometry analysis of PBMC stained with APC-iFluor® 700 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 700 specific R4-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fapc-ifluor-700-tandem%2Ffigure-for-apc-ifluor-700-tandem_ow1d9.png&w=640&q=75)
![Flow cytometry analysis of PBMC stained with APC-iFluor® 700 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 700 specific R4-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fapc-ifluor-700-tandem%2Ffigure-for-apc-ifluor-700-tandem_ow1d9.png&w=640&q=75)
![Flow cytometry analysis of PBMC stained with APC-iFluor® 700 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 700 specific R4-A channel.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fapc-ifluor-700-tandem%2Ffigure-for-apc-ifluor-700-tandem_ow1d9.png&w=128&q=25)
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
APC-iFluor® 750 Tandem | 651 | 776 | 700000 |
APC-iFluor® 800 Tandem | 651 | 819 | 700000 |
APC-iFluor® 710 Tandem | 651 | 747 | 700000 |
APC-iFluor®A7 Tandem | 651 | 782 | 700000 |
APC-iFluor® 720 Tandem | 651 | 748 | 700000 |
APC-iFluor® 740 Tandem | 651 | 764 | 700000 |
APC-iFluor® 770 Tandem | 780 | 800 | 700000 |
APC-iFluor® 780 Tandem | 787 | 812 | 700000 |
PE-iFluor® 700 Tandem | 565 | 708 | 1960000 |
References
View all 2 references: Citation Explorer
Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
Cy7PE and Cy7APC: bright new probes for immunofluorescence.
Authors: Roederer, M and Kantor, A B and Parks, D R and Herzenberg, L A
Journal: Cytometry (1996): 191-7
Authors: Roederer, M and Kantor, A B and Parks, D R and Herzenberg, L A
Journal: Cytometry (1996): 191-7