APC-iFluor® 700 Tandem
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Solvent | Water |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 700000 |
| Excitation (nm) | 651 |
| Emission (nm) | 710 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| APC-iFluor® 750-streptavidin conjugate |
| Overview |  SDSProtocol |
Extinction coefficient (cm -1 M -1) 700000 | Excitation (nm) 651 | Emission (nm) 710 |
Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. Its strong absorption at 633 nm and 647 nm makes the tandem one of the best fluorophores used with a red laser in flow cytometry applications. APC-XFD 700 has the same structure to Alexa Fluor® 700 tandem. It is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at ~710 nm.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 700000 |
| Excitation (nm) | 651 |
| Emission (nm) | 710 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| APC-iFluor® 750 Tandem | 651 | 776 | 700000 |
| APC-iFluor® 800 Tandem | 651 | 819 | 700000 |
| APC-iFluor® 710 Tandem | 651 | 747 | 700000 |
| APC-iFluor®A7 Tandem | 651 | 782 | 700000 |
| APC-iFluor® 720 Tandem | 651 | 748 | 700000 |
| APC-iFluor® 740 Tandem | 651 | 764 | 700000 |
| APC-iFluor® 770 Tandem | 780 | 800 | 700000 |
| APC-iFluor® 780 Tandem | 787 | 812 | 700000 |
| PE-iFluor® 700 Tandem | 565 | 708 | 1960000 |
Images
Figure 1. Flow cytometry analysis of PBMC stained with APC-iFluor® 700 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 700 specific R4-A channel.
References
View all 2 references: Citation Explorer
Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
Cy7PE and Cy7APC: bright new probes for immunofluorescence.
Authors: Roederer, M and Kantor, A B and Parks, D R and Herzenberg, L A
Journal: Cytometry (1996): 191-7
Authors: Roederer, M and Kantor, A B and Parks, D R and Herzenberg, L A
Journal: Cytometry (1996): 191-7