logo
AAT Bioquest

APC-iFluor® 700 Tandem

Flow cytometry analysis of PBMC stained with APC-iFluor® 700 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 700 specific R4-A channel.
Flow cytometry analysis of PBMC stained with APC-iFluor® 700 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 700 specific R4-A channel.
Flow cytometry analysis of PBMC stained with APC-iFluor® 700 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 700 specific R4-A channel.
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)710
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Extinction coefficient (cm -1 M -1)
700000
Excitation (nm)
651
Emission (nm)
710
Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. Its strong absorption at 633 nm and 647 nm makes the tandem one of the best fluorophores used with a red laser in flow cytometry applications. APC-XFD 700 has the same structure to Alexa Fluor® 700 tandem. It is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at ~710 nm.

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)710

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
APC-iFluor® 750 Tandem651776700000
APC-iFluor® 800 Tandem651819700000
APC-iFluor® 710 Tandem651747700000
APC-iFluor®A7 Tandem651782700000
APC-iFluor® 720 Tandem651748700000
APC-iFluor® 740 Tandem651764700000
APC-iFluor® 770 Tandem780800700000
APC-iFluor® 780 Tandem787812700000
PE-iFluor® 700 Tandem5657081960000

Images


References


View all 2 references: Citation Explorer
Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
Cy7PE and Cy7APC: bright new probes for immunofluorescence.
Authors: Roederer, M and Kantor, A B and Parks, D R and Herzenberg, L A
Journal: Cytometry (1996): 191-7