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APC-iFluor® 710 Tandem

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). APC-iFluor® 710 is a new unique tandem color for multicolor applications, in particular, for designing spectral flow cytometric panels. It has been validated with Cytek’s Aurora flow cytometer. Its primary absorption peak is at 651 nm with emission peak at ~740 nm. It is one of the brightest NIR fluorophores that can be used with flow cytometry and fluorescence imaging applications.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
APC-iFluor® 700 Tandem651710700000
APC-iFluor® 750 Tandem651776700000
APC-iFluor® 800 Tandem651819700000
APC-iFluor®A7 Tandem651782700000
APC-iFluor® 720 Tandem651748700000
APC-iFluor® 740 Tandem651764700000
APC-iFluor® 770 Tandem780800700000
APC-iFluor® 780 Tandem787812700000
PE-iFluor® 710 Tandem5657471960000

References

View all 8 references: Citation Explorer
Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor.
Authors: Rudkouskaya, Alena and Sinsuebphon, Nattawut and Ochoa, Marien and Chen, Sez-Jade and Mazurkiewicz, Joseph E and Intes, Xavier and Barroso, Margarida
Journal: Theranostics (2020): 10309-10325
Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents.
Authors: Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel
Journal: Biomedical optics express (2018): 2229-2239
An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots.
Authors: Samanta, Anirban and Walper, Scott A and Susumu, Kimihiro and Dwyer, Chris L and Medintz, Igor L
Journal: Nanoscale (2015): 7603-14
Multicolor detection of rare tumor cells in blood using a novel flow cytometry-based system.
Authors: Watanabe, Masaru and Uehara, Yuri and Yamashita, Namiko and Fujimura, Yuu and Nishio, Kaori and Sawada, Takeshi and Takeda, Kazuo and Koizumi, Fumiaki and Koh, Yasuhiro
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2014): 206-13
Noninvasive and quantitative assessment of in vivo fetomaternal interface angiogenesis using RGD-based fluorescence.
Authors: Keramidas, M and Lavaud, J and Sergent, F and Hoffmann, P and Brouillet, S and Feige, J-J and Coll, J-L and Alfaidy, N
Journal: BioMed research international (2014): 309082
Page updated on December 6, 2024

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Catalog Number2631
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Physical properties

Molecular weight

N/A

Solvent

Water

Spectral properties

Correction Factor (280 nm)

0.16

Extinction coefficient (cm -1 M -1)

700000

Excitation (nm)

651

Emission (nm)

747

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
Flow cytometry analysis of PBMC stained with APC-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 710 specific R6-A channel.
Flow cytometry analysis of PBMC stained with APC-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 710 specific R6-A channel.
Flow cytometry analysis of PBMC stained with APC-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 710 specific R6-A channel.