APC-iFluor® 710 Tandem

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	Water

Spectral properties

Correction Factor (280 nm)	0.16
Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	747

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

N/A

Correction Factor (280 nm)

0.16

Extinction coefficient (cm ^-1 M ^-1)

700000

Excitation (nm)

651

Emission (nm)

747

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). APC-iFluor® 710 is a new unique tandem color for multicolor applications, in particular, for designing spectral flow cytometric panels. It has been validated with Cytek’s Aurora flow cytometer. Its primary absorption peak is at 651 nm with emission peak at ~740 nm. It is one of the brightest NIR fluorophores that can be used with flow cytometry and fluorescence imaging applications.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (280 nm)	0.16
Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	747

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
APC-iFluor® 700 Tandem	651	710	700000
APC-iFluor® 750 Tandem	651	776	700000
APC-iFluor® 800 Tandem	651	819	700000
APC-iFluor®A7 Tandem	651	782	700000
APC-iFluor® 720 Tandem	651	748	700000
APC-iFluor® 740 Tandem	651	764	700000
APC-iFluor® 770 Tandem	780	800	700000
APC-iFluor® 780 Tandem	787	812	700000
PE-iFluor® 710 Tandem	565	747	1960000

Images

Figure 1. Flow cytometry analysis of PBMC stained with APC-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 710 specific R6-A channel.

References

View all 8 references: Citation Explorer

Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor.
Authors: Rudkouskaya, Alena and Sinsuebphon, Nattawut and Ochoa, Marien and Chen, Sez-Jade and Mazurkiewicz, Joseph E and Intes, Xavier and Barroso, Margarida
Journal: Theranostics (2020): 10309-10325

Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents.
Authors: Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel
Journal: Biomedical optics express (2018): 2229-2239

An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots.
Authors: Samanta, Anirban and Walper, Scott A and Susumu, Kimihiro and Dwyer, Chris L and Medintz, Igor L
Journal: Nanoscale (2015): 7603-14

Noninvasive and quantitative assessment of in vivo fetomaternal interface angiogenesis using RGD-based fluorescence.
Authors: Keramidas, M and Lavaud, J and Sergent, F and Hoffmann, P and Brouillet, S and Feige, J-J and Coll, J-L and Alfaidy, N
Journal: BioMed research international (2014): 309082

Multicolor detection of rare tumor cells in blood using a novel flow cytometry-based system.
Authors: Watanabe, Masaru and Uehara, Yuri and Yamashita, Namiko and Fujimura, Yuu and Nishio, Kaori and Sawada, Takeshi and Takeda, Kazuo and Koizumi, Fumiaki and Koh, Yasuhiro
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2014): 206-13

Intraoperative near-infrared image-guided surgery for peritoneal carcinomatosis in a preclinical experimental model.
Authors: Keramidas, M and Josserand, V and Righini, C A and Wenk, C and Faure, C and Coll, J L
Journal: The British journal of surgery (2010): 737-43

Homogeneous TR-FRET high-throughput screening assay for calcium-dependent multimerization of sorcin.
Authors: Appelblom, Heidi and Nurmi, Jussi and Soukka, Tero and Pasternack, Michael and Penttilä, Kai E and Lövgren, Timo and Niemelä, Pauliina
Journal: Journal of biomolecular screening (2007): 842-8

Analysis of hollow-core photonic bandgap fibers for evanescent wave biosensing.
Authors: Sun, Jian and Chan, Chi-Chiu and Zhang, Yi-Fan and Shum, Ping
Journal: Journal of biomedical optics : 054048