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APC-iFluor® 720 Tandem

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). APC-iFluor® 720 is a new unique tandem color for multicolor applications, in particular, for designing spectral flow cytometric panels. It has been validated with Cytek’s Aurora flow cytometer. Its primary absorption peak is at 651 nm with emission peak at ~740 nm. It is one of the brightest NIR fluorophores that can be used with flow cytometry and fluorescence imaging applications.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
APC-iFluor® 700 Tandem651710700000
APC-iFluor® 750 Tandem651776700000
APC-iFluor® 800 Tandem651819700000
APC-iFluor® 710 Tandem651747700000
APC-iFluor®A7 Tandem651782700000
APC-iFluor® 740 Tandem651764700000
APC-iFluor® 770 Tandem780800700000
APC-iFluor® 780 Tandem787812700000
PE-iFluor® 720 Tandem5657501960000

References

View all 6 references: Citation Explorer
iCoreDrop: A robust immune monitoring spectral cytometry assay with six open channels for biomarker flexibility.
Authors: Jensen, Holly A and Kim, Jeong
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2022)
Comparison between photostability of Alexa Fluor 448 and Alexa Fluor 647 with conventional dyes FITC and APC by flow cytometry.
Authors: Rai, S and Bhardwaj, U and Misra, A and Singh, S and Gupta, R
Journal: International journal of laboratory hematology (2018): e52-e54
[Changes of monocyte and monocyte-platelet aggregates in different subgroups of thrombotic events in patients with acute myocardial infarction during PCI].
Authors: Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965
Dual-Color Fluorescence Imaging of EpCAM and EGFR in Breast Cancer Cells with a Bull's Eye-Type Plasmonic Chip.
Authors: Izumi, Shota and Yamamura, Shohei and Hayashi, Naoko and Toma, Mana and Tawa, Keiko
Journal: Sensors (Basel, Switzerland) (2017)
Antigen processing of glycoconjugate vaccines; the polysaccharide portion of the pneumococcal CRM(197) conjugate vaccine co-localizes with MHC II on the antigen processing cell surface.
Authors: Lai, Zengzu and Schreiber, John R
Journal: Vaccine (2009): 3137-44
Page updated on December 13, 2024

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Catalog Number2633
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Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm -1 M -1)

700000

Excitation (nm)

651

Emission (nm)

748

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
Flow cytometry analysis of PBMC stained with APC-iFluor® 720 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 720 specific R6-A channel.
Flow cytometry analysis of PBMC stained with APC-iFluor® 720 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 720 specific R6-A channel.
Flow cytometry analysis of PBMC stained with APC-iFluor® 720 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 720 specific R6-A channel.