APC-XFD700 Tandem

Allophycocyanin (APC) is a phycobiliprotein with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. Its strong absorption at 633 nm and 647 nm makes the APC tandem one of the best fluorophores used with a red laser in flow cytometry applications. Compared to the commonly used APC-Alexa Fluor® 700, APC-XFD 700 gives significantly improved stain index for certain CD antibodies than APC-Alexa Fluor® 700 tandem under the same conditions. Its primary absorption peak is at 651 nm with emission peak at ~710 nm. Due to their similar spectral properties, APC-XFD 700 Tandem-labeled antibodies can be used to replace the corresponding APC-Alexa Fluor® 700 antibody conjugates.

Flow cytometry analysis of PBMC stained with APC-XFD700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-XFD700 specific R6-A channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2624	1 mg	Price

References

View all 8 references: Citation Explorer

Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor.

Authors:

Rudkouskaya, Alena and Sinsuebphon, Nattawut and Ochoa, Marien and Chen, Sez-Jade and Mazurkiewicz, Joseph E and Intes, Xavier and Barroso, Margarida

Journal:

Theranostics (2020): 10309-10325

Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents.

Authors:

Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel

Journal:

Biomedical optics express (2018): 2229-2239

An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots.

Authors:

Samanta, Anirban and Walper, Scott A and Susumu, Kimihiro and Dwyer, Chris L and Medintz, Igor L

Journal:

Nanoscale (2015): 7603-14

Multicolor detection of rare tumor cells in blood using a novel flow cytometry-based system.

Authors:

Watanabe, Masaru and Uehara, Yuri and Yamashita, Namiko and Fujimura, Yuu and Nishio, Kaori and Sawada, Takeshi and Takeda, Kazuo and Koizumi, Fumiaki and Koh, Yasuhiro

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2014): 206-13

Noninvasive and quantitative assessment of in vivo fetomaternal interface angiogenesis using RGD-based fluorescence.

Authors:

Keramidas, M and Lavaud, J and Sergent, F and Hoffmann, P and Brouillet, S and Feige, J-J and Coll, J-L and Alfaidy, N

Journal:

BioMed research international (2014): 309082

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	651
Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	707

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 21, 2025