APC-XFD700 Tandem

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	651
Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	707

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

Direct upgrades

Overview SDS Protocol

Upgrade: APC-iFluor® 700 Tandem

Absorbance (nm)

651

Extinction coefficient (cm ^-1 M ^-1)

700000

Excitation (nm)

651

Emission (nm)

707

Allophycocyanin (APC) is a phycobiliprotein with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. Its strong absorption at 633 nm and 647 nm makes the APC tandem one of the best fluorophores used with a red laser in flow cytometry applications. Compared to the commonly used APC-Alexa Fluor® 700, APC-XFD 700 gives significantly improved stain index for certain CD antibodies than APC-Alexa Fluor® 700 tandem under the same conditions. Its primary absorption peak is at 651 nm with emission peak at ~710 nm. Due to their similar spectral properties, APC-XFD 700 Tandem-labeled antibodies can be used to replace the corresponding APC-Alexa Fluor® 700 antibody conjugates.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	651
Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	707

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
APC-Cy7 Tandem	651	779	700000
APC-XFD750 Tandem	651	776	700000

Images

Figure 1. Flow cytometry analysis of PBMC stained with APC-XFD700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-XFD700 specific R6-A channel.

References

View all 8 references: Citation Explorer

Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor.
Authors: Rudkouskaya, Alena and Sinsuebphon, Nattawut and Ochoa, Marien and Chen, Sez-Jade and Mazurkiewicz, Joseph E and Intes, Xavier and Barroso, Margarida
Journal: Theranostics (2020): 10309-10325

Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents.
Authors: Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel
Journal: Biomedical optics express (2018): 2229-2239

An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots.
Authors: Samanta, Anirban and Walper, Scott A and Susumu, Kimihiro and Dwyer, Chris L and Medintz, Igor L
Journal: Nanoscale (2015): 7603-14

Noninvasive and quantitative assessment of in vivo fetomaternal interface angiogenesis using RGD-based fluorescence.
Authors: Keramidas, M and Lavaud, J and Sergent, F and Hoffmann, P and Brouillet, S and Feige, J-J and Coll, J-L and Alfaidy, N
Journal: BioMed research international (2014): 309082

Multicolor detection of rare tumor cells in blood using a novel flow cytometry-based system.
Authors: Watanabe, Masaru and Uehara, Yuri and Yamashita, Namiko and Fujimura, Yuu and Nishio, Kaori and Sawada, Takeshi and Takeda, Kazuo and Koizumi, Fumiaki and Koh, Yasuhiro
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2014): 206-13

Intraoperative near-infrared image-guided surgery for peritoneal carcinomatosis in a preclinical experimental model.
Authors: Keramidas, M and Josserand, V and Righini, C A and Wenk, C and Faure, C and Coll, J L
Journal: The British journal of surgery (2010): 737-43

Homogeneous TR-FRET high-throughput screening assay for calcium-dependent multimerization of sorcin.
Authors: Appelblom, Heidi and Nurmi, Jussi and Soukka, Tero and Pasternack, Michael and Penttilä, Kai E and Lövgren, Timo and Niemelä, Pauliina
Journal: Journal of biomolecular screening (2007): 842-8

Analysis of hollow-core photonic bandgap fibers for evanescent wave biosensing.
Authors: Sun, Jian and Chan, Chi-Chiu and Zhang, Yi-Fan and Shum, Ping
Journal: Journal of biomedical optics : 054048