logo
AAT Bioquest

APC-XFD700 Tandem

Allophycocyanin (APC) is a phycobiliprotein with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. Its strong absorption at 633 nm and 647 nm makes the APC tandem one of the best fluorophores used with a red laser in flow cytometry applications. Compared to the commonly used APC-Alexa Fluor® 700, APC-XFD 700 gives significantly improved stain index for certain CD antibodies than APC-Alexa Fluor® 700 tandem under the same conditions. Its primary absorption peak is at 651 nm with emission peak at ~710 nm. Due to their similar spectral properties, APC-XFD 700 Tandem-labeled antibodies can be used to replace the corresponding APC-Alexa Fluor® 700 antibody conjugates.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
APC-Cy7 Tandem651779700000
APC-XFD750 Tandem651776700000

References

View all 8 references: Citation Explorer
Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor.
Authors: Rudkouskaya, Alena and Sinsuebphon, Nattawut and Ochoa, Marien and Chen, Sez-Jade and Mazurkiewicz, Joseph E and Intes, Xavier and Barroso, Margarida
Journal: Theranostics (2020): 10309-10325
Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents.
Authors: Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel
Journal: Biomedical optics express (2018): 2229-2239
An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots.
Authors: Samanta, Anirban and Walper, Scott A and Susumu, Kimihiro and Dwyer, Chris L and Medintz, Igor L
Journal: Nanoscale (2015): 7603-14
Multicolor detection of rare tumor cells in blood using a novel flow cytometry-based system.
Authors: Watanabe, Masaru and Uehara, Yuri and Yamashita, Namiko and Fujimura, Yuu and Nishio, Kaori and Sawada, Takeshi and Takeda, Kazuo and Koizumi, Fumiaki and Koh, Yasuhiro
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2014): 206-13
Noninvasive and quantitative assessment of in vivo fetomaternal interface angiogenesis using RGD-based fluorescence.
Authors: Keramidas, M and Lavaud, J and Sergent, F and Hoffmann, P and Brouillet, S and Feige, J-J and Coll, J-L and Alfaidy, N
Journal: BioMed research international (2014): 309082
Page updated on December 6, 2024

Ordering information

Price
Unit size
Catalog Number2624
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)

651

Extinction coefficient (cm -1 M -1)

700000

Excitation (nm)

651

Emission (nm)

707

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Flow cytometry analysis of PBMC stained with APC-XFD700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-XFD700 specific R6-A channel.
Flow cytometry analysis of PBMC stained with APC-XFD700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-XFD700 specific R6-A channel.
Flow cytometry analysis of PBMC stained with APC-XFD700 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-XFD700 specific R6-A channel.