Name: APTS [8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt] *CAS 196504-57-1*
Brand: AAT Bioquest
SKU: 624
Price: 90 USD
Availability: InStock

APTS

8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt; CAS 196504-57-1

This water-soluble reagent has strong green fluorescence with great Stokes shift. It is widely used for detecting carbohydrates and labeling glycoproteins in electrophoresis. It is also used to modify other carbonyl-containing biomolecules such as aldehydes and ketones.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
624	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of APTS [8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt] *CAS 196504-57-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	191.058 µL	955.292 µL	1.911 mL	9.553 mL	19.106 mL
5 mM	38.212 µL	191.058 µL	382.117 µL	1.911 mL	3.821 mL
10 mM	19.106 µL	95.529 µL	191.058 µL	955.292 µL	1.911 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	523.4 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

A rapid and facile preparation of APTS-labeled N-glycans by combination of ion pair-assisted extraction and HILIC-SPE for routine glycan analysis

Authors:

Kinoshita, Mitsuhiro and Nakatani, Yumi and Yamada, Keita and Yamamoto, Sachio and Suzuki, Shigeo

Journal:

Journal of Pharmaceutical and Biomedical Analysis (2021): 113875

High-throughput N-glycan screening method for therapeutic antibodies using a microchip-based DNA analyzer: a promising methodology for monitoring monoclonal antibody N-glycosylation

Authors:

Kinoshita, Mitsuhiro and Nakajima, Kazuki and Yamamoto, Sachio and Suzuki, Shigeo

Journal:

Analytical and Bioanalytical Chemistry (2021): 1--12

References

View all 28 references: Citation Explorer

A novel tool to characterize paracellular transport: the APTS-dextran ladder

Authors:

Neuhaus W, Bogner E, Wirth M, Trzeciak J, Lachmann B, Gabor F, Noe CR.

Journal:

Pharm Res (2006): 1491

Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides

Authors:

Kabel MA, Heijnis WH, Bakx EJ, Kuijpers R, Voragen AG, Schols HA.

Journal:

J Chromatogr A. (2006)

A novel ultra-sensitive method for the quantification of glycosaminoglycan disaccharides using an automated DNA sequencer

Authors:

Vogel K, Kuhn J, Kleesiek K, Gotting C.

Journal:

Electrophoresis (2006): 1363

Capillary electrophoresis-mass spectrometry as a characterization tool for therapeutic proteins

Authors:

Gennaro LA, Salas-Solano O, Ma S.

Journal:

Anal Biochem (2006): 249

APTS-labeled dextran ladder: a novel tool to characterize cell layer tightness

Authors:

Neuhaus W, Trzeciak J, Lauer R, Lachmann B, Noe CR.

Journal:

J Pharm Biomed Anal (2006): 1035

Physical properties

Molecular weight	523.40
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	17160
Excitation (nm)	427
Emission (nm)	478

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	196504-57-1

Instrument settings

Fluorescence microplate reader
Excitation	424 nm
Emission	505 nm
Cutoff	450 nm
Recommended plate	Solid black

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Page updated on October 1, 2025