ATP-TAMRA conjugate [5-TAMRA-eda-ATP]

ATP-TAMRA conjugate

5-TAMRA-eda-ATP

Fluorescently labeled ATP tracers are useful for monitoring ATP-binding proteins and other biomolecules. The ATP-TAMRA tracer was demonstrated to bind ATPases that are a class of enzymes that catalyze the decomposition of ATP into ADP and a free phosphate ion.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13607	100 nmol	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ATP-TAMRA conjugate [5-TAMRA-eda-ATP] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	88.642 µL	443.211 µL	886.423 µL	4.432 mL	8.864 mL
5 mM	17.728 µL	88.642 µL	177.285 µL	886.423 µL	1.773 mL
10 mM	8.864 µL	44.321 µL	88.642 µL	443.211 µL	886.423 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1128.13 g/mol	=	mL	x	mM	=	mmol

References

View all 32 references: Citation Explorer

Deciphering the catalysis-associated conformational changes of human adenylate kinase 1 with single-molecule spectroscopy

Authors:

Lin CY, Huang JY, Lo LW.

Journal:

J Phys Chem B (2013): 13947

The Escherichia coli PriA helicase-double-stranded DNA complex: location of the strong DNA-binding subsite on the helicase domain of the protein and the affinity control by the two nucleotide-binding sites of the enzyme

Authors:

Szymanski MR, Jezewska MJ, Bujalowski W.

Journal:

J Mol Biol (2010): 344

The effect of NBD-Cl in nucleotide-binding of the major subunit alpha and B of the motor proteins F1FO ATP synthase and A1AO ATP synthase

Authors:

Hunke C, Tadwal VS, Manimekalai MS, Roessle M, Gruber G.

Journal:

J Bioenerg Biomembr (2010): 1

ATP/ADP binding to a novel nucleotide binding domain of the reticulocyte-binding protein Py235 of Plasmodium yoelii

Authors:

Ramalingam JK, Hunke C, Gao X, Gruber G, Preiser PR.

Journal:

J Biol Chem (2008): 36386

Reversal of ADP-mediated aggregation of adenosine kinase by cyclophilin leads to its reactivation

Authors:

Sen B, Chakraborty A, Datta R, Bhattacharyya D, Datta AK.

Journal:

Biochemistry (2006): 263

Physical properties

Molecular weight	1128.13
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on July 12, 2023