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ATTO 488 TCO

ATTO 488 is a hydrophilic, rhodamine-based fluorescent dye with exceptional water solubility. It is characterized by strong absorption, a high fluorescence quantum yield, and exceptional photostability, making it highly suitable for advanced fluorescence imaging techniques. The dye exhibits optimal excitation within the 480-515 nm wavelength range, aligning precisely with the 488 nm emission line of the Argon-Ion laser. ATTO 488 is particularly effective for single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. Moreover, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other bioanalytical applications.

ATTO 488 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ATTO 488 acid499520900000.800.220.09
iFluor® 488 TCO4915167500010.910.210.11
ATTO 488 maleimide499520900000.800.220.09
ATTO 488 azide499520900000.800.220.09
ATTO 488 alkyne499520900000.800.220.09
ATTO 488 DBCO499520900000.800.220.09
ATTO 390 TCO39047524000.900.460.09
ATTO 425 TCO438484450000.900.190.17
ATTO 495 TCO497525800000.20.450.37
ATTO 550 TCO5535741200000.800.230.10
ATTO 590 TCO5926211200000.800.390.43
ATTO 610 TCO6156321500000.700.030.06
ATTO 620 TCO61964112000010.510.040.06
ATTO 633 TCO6296511300000.6410.040.05
ATTO 655 TCO6616791250000.310.240.08
ATTO 488 Tetrazine499520900000.800.220.09
ATTO 647N TCO6456631500000.6510.060.05
ATTO 680 TCO6796961250000.300.300.17
ATTO 700 TCO6997151200000.250.260.41
ATTO 594 TCO6026211200000.850.260.51
Show More (11)

References

View all 32 references: Citation Explorer
Time-resolved fluorescence anisotropy with Atto 488-labeled phytochrome Agp1 from Agrobacterium fabrum.
Authors: Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman
Journal: Photochemistry and photobiology (2024): 561-572
Analysis of photon trajectories from diffusing single molecules.
Authors: Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung
Journal: The Journal of chemical physics (2023)
Curvature preference of cubic CsPbBr3 quantum dots embedded onto phospholipid bilayer membranes.
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
Interactions of the Kv1.1 Channel with Peptide Pore Blockers: A Fluorescent Analysis on Mammalian Cells.
Authors: Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V
Journal: Membranes (2023)
Spatial distribution of single guest molecules along thickness of thin films of poly(2-hydroxyethyl acrylate).
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184
Page updated on October 12, 2024

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Catalog Number2801
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Physical properties

Molecular weight

899.09

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.22

Correction Factor (280 nm)

0.09

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

499

Emission (nm)

520

Quantum yield

0.80

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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