ATTO 488 TCO
ATTO 488 is a hydrophilic, rhodamine-based fluorescent dye with exceptional water solubility. It is characterized by strong absorption, a high fluorescence quantum yield, and exceptional photostability, making it highly suitable for advanced fluorescence imaging techniques. The dye exhibits optimal excitation within the 480-515 nm wavelength range, aligning precisely with the 488 nm emission line of the Argon-Ion laser. ATTO 488 is particularly effective for single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. Moreover, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other bioanalytical applications.
ATTO 488 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ATTO 488 acid | 499 | 520 | 90000 | 0.80 | 0.22 | 0.09 |
iFluor® 488 TCO | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
ATTO 488 maleimide | 499 | 520 | 90000 | 0.80 | 0.22 | 0.09 |
ATTO 488 azide | 499 | 520 | 90000 | 0.80 | 0.22 | 0.09 |
ATTO 488 alkyne | 499 | 520 | 90000 | 0.80 | 0.22 | 0.09 |
ATTO 488 DBCO | 499 | 520 | 90000 | 0.80 | 0.22 | 0.09 |
ATTO 390 TCO | 390 | 475 | 24000 | .90 | 0.46 | 0.09 |
ATTO 425 TCO | 438 | 484 | 45000 | 0.90 | 0.19 | 0.17 |
ATTO 495 TCO | 497 | 525 | 80000 | 0.2 | 0.45 | 0.37 |
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References
Authors: Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman
Journal: Photochemistry and photobiology (2024): 561-572
Authors: Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung
Journal: The Journal of chemical physics (2023)
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
Authors: Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V
Journal: Membranes (2023)
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184