ATTO 565 NHS ester

ATTO 565 NHS ester is an amine‑reactive, orange fluorescent dye with an excitation/emission of 562/589 nm that is used for FACS and FISH applications.

Amine‑Specific Conjugation: Forms stable amide bonds with primary amines on peptides and amine‑modified oligonucleotides
High Quantum Yield & Photostability: Delivers bright, dependable fluorescence
Ideal for Super‑Resolution Imaging: Compatible with PALM, dSTORM, and STED

ATTO 565 acid is a commonly used amine-reactive rhodamine dye.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2828	5 mg	Price

Overview

ATTO 565 NHS ester is manufactured by AAT Bioquest for research and development use. ATTO 565 NHS ester is a commonly used amine-reactive rhodamine dye. It might be used for labeling peptides and amino-modified oligonucleotides. It readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye and amino compounds. The optimum pH range for NHS-ester coupling is pH 8.0 – 9.0. At this pH, amino groups of peptides (such as the ε-amino groups of lysines) are unprotonated enough for fast coupling. ATTO 565 NHS ester has strong absorption, high fluorescence quantum yield and high thermal/photostability. However, it has low water solubility. For the applications that require high water solubility, you may consider iFluor® 568 succinimidyl ester that has almost identical fluorescence properties with greatly enhanced water solubility. In addition, the protein conjugates made from iFluor® 568 succinimidyl ester are much brighter than those from ATTO 565 NHS ester.

References

View all 50 references: Citation Explorer

Time-resolved fluorescence anisotropy with Atto 488-labeled phytochrome Agp1 from Agrobacterium fabrum.

Authors:

Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman

Journal:

Photochemistry and photobiology (2024): 561-572

Fluorescence quenching-based immunological probe for ticagrelor monitoring.

Authors:

Zhang, Shengshuo and Cheng, Yueqing and Gao, Yujie and Zou, Yujie and Xiao, Weiling and Li, Tianyi and Li, Mei and Yu, Bowen and Dong, Jinhua

Journal:

Frontiers in bioengineering and biotechnology (2023): 1295406

Interactions of the Kv1.1 Channel with Peptide Pore Blockers: A Fluorescent Analysis on Mammalian Cells.

Authors:

Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V

Journal:

Membranes (2023)

Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells.

Authors:

Xiao, Wen and Yeom, Kyu-Hyeon and Lin, Chia-Ho and Black, Douglas L

Journal:

RNA (New York, N.Y.) (2023)

Measuring Photophysical Transition Rates with Fluorescence Correlation Spectroscopy and Antibunching.

Authors:

Sakhapov, Damir and Gregor, Ingo and Karedla, Narain and Enderlein, Jörg

Journal:

The journal of physical chemistry letters (2022): 4823-4830

Physical properties

Molecular weight	708.12
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.27
Correction factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	120000
Excitation (nm)	562
Emission (nm)	589
Quantum yield	0.90

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 25, 2025