ATTO 590 NHS ester is manufactured by AAT Bioquest for research and development use. ATTO 590 is a rhodamine-based fluorescent dye characterized by its strong absorption, high fluorescence quantum yield, and excellent photostability and thermal stability. It exhibits moderate hydrophilicity and is optimally excited within the 575-610 nm wavelength range. ATTO 590 emits in the orange-red region of the visible spectrum, with fluorescence effectively quenched by BHQ®-2 dye. This dye is particularly suited for advanced applications in single-molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED microscopy. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays. ATTO 590 is a suitable alternative to Alexa Fluor® 594 for these applications.

NHS esters react selectively and efficiently with primary amines (such as the side chains of lysine residues or aminosilane-coated surfaces) at pH 7-9, forming stable covalent amide bonds. This property makes ATTO 590 NHS ester an excellent choice for labeling peptides and amine-modified oligonucleotides. For labeling antibodies and other proteins, we highly recommend our iFluor® dyes that are highly water soluble and are optimized for labeling proteins.