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ATTO 620 Tetrazine

Product key features

  • Ex/Em: 619/641 nm
  • Extinction coefficient: 120,000 cm-1M-1
  • Reactive group: Tetrazine
  • Rapid Conjugation: Ideal for labeling TCO-modified biomolecules under copper-free conditions, providing fast reaction kinetics
  • High Quantum Yield & Stability: Delivers bright, stable fluorescence under prolonged light exposure, temperature changes, and across pH 2–11
  • Temperature-Dependent Fluorescence: Enables precise thermal mapping in live cells, protein studies, and reaction monitoring

Product description

ATTO 620 is a bright-red fluorescent dye, characterized by its strong absorption, exceptional photo and thermal stability, and temperature-dependent fluorescence. It demonstrates moderate hydrophilicity and optimal excitation within the 600-630 nm wavelength range. The dye maintains stable fluorescence over a wide pH range (2-11), allowing for its use in diverse experimental conditions. Upon conjugation to a substrate, ATTO 620 becomes cationic, carrying a net positive charge of +1. These properties make ATTO 620 particularly suitable for high-precision applications, including single-molecule detection and super-resolution microscopy techniques such as PALM, dSTORM, and STED. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

ATTO 620 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ATTO 390 Tetrazine39047524000.900.460.09
ATTO 425 Tetrazine438484450000.900.190.17
ATTO 495 Tetrazine497525800000.20.450.37
ATTO 550 Tetrazine5535741200000.800.230.10
ATTO 590 Tetrazine5926211200000.800.390.43
ATTO 610 Tetrazine6156321500000.700.030.06
ATTO 620 acid61964112000010.510.040.06
ATTO 620 maleimide61964112000010.510.040.06
ATTO 620 azide61964112000010.510.040.06
ATTO 620 alkyne61964112000010.510.040.06
ATTO 620 DBCO61964112000010.510.040.06
ATTO 620 TCO61964112000010.510.040.06
ATTO 633 Tetrazine6296511300000.6410.040.05
ATTO 655 Tetrazine6616791250000.310.240.08
ATTO 488 Tetrazine499520900000.800.220.09
ATTO 647N Tetrazine6456631500000.6510.060.05
ATTO 680 Tetrazine6796961250000.300.300.17
ATTO 700 Tetrazine6997151200000.250.260.41
ATTO 647 Tetrazine6466661200000.200.080.04
ATTO 594 Tetrazine6026211200000.850.260.51
ATTO 532 Tetrazine5315521150000.900.220.11
Show More (12)

References

View all 4 references: Citation Explorer
Two-Photon-Excited Single-Molecule Fluorescence Enhanced by Gold Nanorod Dimers.
Authors: Lu, Xuxing and Punj, Deep and Orrit, Michel
Journal: Nano letters (2022): 4215-4222
Ultrabright Terbium Nanoparticles for FRET Biosensing and in Situ Imaging of Epidermal Growth Factor Receptors*.
Authors: Charpentier, Cyrille and Cifliku, Vjona and Goetz, Joan and Nonat, Aline and Cheignon, Clémence and Cardoso Dos Santos, Marcelina and Francés-Soriano, Laura and Wong, Ka-Leung and Charbonnière, Loïc J and Hildebrandt, Niko
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 14602-14611
Oxazine dye-conjugated dna oligonucleotides: Förster resonance energy transfer in view of molecular dye-DNA interactions.
Authors: Kupstat, Annette and Ritschel, Thomas and Kumke, Michael U
Journal: Bioconjugate chemistry (2011): 2546-57
Novel wavelength-resolved fluorescence detection for a high-throughput capillary electrophoresis system under a diascopic configuration.
Authors: Lin, Shi-Wei and Chang, Guan-Liang and Lin, Che-Hsin
Journal: Journal of chromatography. A (2008): 198-201
Page updated on December 6, 2024

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Catalog Number70267
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Physical properties

Molecular weight

808.91

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.04

Correction Factor (280 nm)

0.06

Extinction coefficient (cm -1 M -1)

1200001

Excitation (nm)

619

Emission (nm)

641

Quantum yield

0.51

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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