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ATTO 647 Tetrazine

Product key features

  • Ex/Em: 646/666 nm
  • Extinction coefficient: 120,000 cm-1M-1
  • Reactive group: Tetrazine
  • Rapid Conjugation: Ideal for labeling TCO-modified biomolecules under copper-free conditions, providing fast reaction kinetics
  • High Quantum Yield & Stability: Maintains bright fluorescence under prolonged light exposure and stable across pH 2–11
  • Zwitterionic & Hydrophilic: Minimizes non-specific binding and dissolves easily in aqueous environments for optimal biological assays and live-cell imaging

Product description

ATTO 647 is a rhodamine-derived fluorescent dye optimized for applications within the red spectral region, offering spectral characteristics similar to Cy5. Its characterized by a high molar absorptivity, robust fluorescence quantum yield, high photostability, and good aqueous solubility, making it well-suited for demanding experimental conditions. The dye is highly hydrophilic, with an excitation maximum between the 615-660 nm range, making it compatible with the 633 nm He:Ne laser, 647 nm Krypton-Ion laser, and 650 nm diode laser. As a zwitterionic molecule, ATTO 647 carries a net neutral charge. The dye is stable under physiological pH conditions and in buffers with a pH of up to 8, though it gradually degrades at higher pH levels. ATTO 647 is ideal for advanced applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays.

ATTO 647 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ATTO 647 acid6466661200000.200.080.04
iFluor® 647 Tetrazine65667025000010.2510.030.03
ATTO 647 maleimide6466661200000.200.080.04
ATTO 390 Tetrazine39047524000.900.460.09
ATTO 425 Tetrazine438484450000.900.190.17
ATTO 495 Tetrazine497525800000.20.450.37
ATTO 550 Tetrazine5535741200000.800.230.10
ATTO 590 Tetrazine5926211200000.800.390.43
ATTO 610 Tetrazine6156321500000.700.030.06
ATTO 620 Tetrazine61964112000010.510.040.06
ATTO 633 Tetrazine6296511300000.6410.040.05
ATTO 655 Tetrazine6616791250000.310.240.08
ATTO 488 Tetrazine499520900000.800.220.09
ATTO 647N Tetrazine6456631500000.6510.060.05
ATTO 680 Tetrazine6796961250000.300.300.17
ATTO 700 Tetrazine6997151200000.250.260.41
ATTO 594 Tetrazine6026211200000.850.260.51
ATTO 532 Tetrazine5315521150000.900.220.11
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Page updated on December 6, 2024

Ordering information

Price
Unit size
Catalog Number2834
Quantity
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Additional ordering information

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Physical properties

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.08

Correction Factor (280 nm)

0.04

Extinction coefficient (cm -1 M -1)

120000

Excitation (nm)

646

Emission (nm)

666

Quantum yield

0.20

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200