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ATTO 700 Tetrazine

Product key features

  • Ex/Em: 699/715 nm
  • Extinction coefficient: 120,000 cm-1M-1
  • Reactive group: Tetrazine
  • Click Chemistry Conjugation: Labels TCO-modified biomolecules under copper-free conditions with fast reaction kinetics
  • High Quantum Yield & Stability: Provides bright fluorescence with high photostability and thermal resilience
  • Zwitterionic: Minimizes non-specific binding to biological surfaces, reducing background noise in live-cell imaging and super-resolution microscopy

Product description

ATTO 700 is a near-infrared fluorescent dye characterized by its strong absorption, high photo and thermal stability, and good aqueous solubility. It is optimally excited within the 670-715 nm wavelength range. As a zwitterionic compound, ATTO 700 remains electrically neutral when conjugated to biomolecules or other substrates. Its strong electron-accepting properties result in efficient fluorescence quenching by electron donors such as guanine and tryptophan. These properties make ATTO 700 ideal for precise applications including single-molecule detection and super-resolution microscopy techniques like PALM, dSTORM, and STED. Furthermore, ATTO 700 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research.

ATTO 700 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ATTO 390 Tetrazine39047524000.900.460.09
ATTO 425 Tetrazine438484450000.900.190.17
ATTO 495 Tetrazine497525800000.20.450.37
ATTO 550 Tetrazine5535741200000.800.230.10
ATTO 590 Tetrazine5926211200000.800.390.43
ATTO 610 Tetrazine6156321500000.700.030.06
ATTO 620 Tetrazine61964112000010.510.040.06
ATTO 633 Tetrazine6296511300000.6410.040.05
ATTO 655 Tetrazine6616791250000.310.240.08
ATTO 488 Tetrazine499520900000.800.220.09
ATTO 647N Tetrazine6456631500000.6510.060.05
ATTO 680 Tetrazine6796961250000.300.300.17
ATTO 700 acid6997151200000.250.260.41
ATTO 700 maleimide6997151200000.250.260.41
ATTO 700 alkyne6997151200000.250.260.41
ATTO 700 TCO6997151200000.250.260.41
ATTO 647 Tetrazine6466661200000.200.080.04
ATTO 594 Tetrazine6026211200000.850.260.51
ATTO 532 Tetrazine5315521150000.900.220.11
Show More (10)

References

View all 6 references: Citation Explorer
Whole blood multiplex measurements using electrochemical aptamer-based biosensors.
Authors: d'Astous, Élodie V and Dauphin-Ducharme, Philippe
Journal: Chemical communications (Cambridge, England) (2024): 6419-6422
Multiphoton absorption enhancement by graphene-gold nanostructure.
Authors: Izadshenas Jahromi, Saeid and Słowik, Karolina
Journal: Optics letters (2024): 3914-3917
Near infrared-emitting multimodal nanosystem for in vitro magnetic hyperthermia of hepatocellular carcinoma and dual imaging of in vivo liver fibrosis.
Authors: Nazeer, Shaiju S and Saraswathy, Ariya and Nimi, Nirmala and Santhakumar, Hema and Radhakrishnapillai Suma, Parvathy and Shenoy, Sachin J and Jayasree, Ramapurath S
Journal: Scientific reports (2023): 12947
Spatial distribution of single guest molecules along thickness of thin films of poly(2-hydroxyethyl acrylate).
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184
Application of single molecule fluorescence microscopy to characterize the penetration of a large amphiphilic molecule in the stratum corneum of human skin.
Authors: Volz, Pierre and Boreham, Alexander and Wolf, Alexander and Kim, Tai-Yang and Balke, Jens and Frombach, Janna and Hadam, Sabrina and Afraz, Zahra and Rancan, Fiorenza and Blume-Peytavi, Ulrike and Vogt, Annika and Alexiev, Ulrike
Journal: International journal of molecular sciences (2015): 6960-77
Page updated on December 6, 2024

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Catalog Number70307
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Physical properties

Molecular weight

862.93

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.26

Correction Factor (280 nm)

0.41

Extinction coefficient (cm -1 M -1)

120000

Excitation (nm)

699

Emission (nm)

715

Quantum yield

0.25

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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