AzoDye-1 Amine
Ordering information
Price | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 475.55 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 522 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
AzoDye-1 Succinimidyl Ester |
AzoDye-1 Maleimide |
AzoDye-1 Azide |
AzoDye-1 Alkyne |
Overview | SDSProtocol |
See also: Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), PCR Detection of Viral DNA/RNA
CAS 1308657-79-5 | Molecular weight 475.55 | Absorbance (nm) 522 |
AzoDye-1 amine is an excellent building block for labeling carbonyl-containing molecules. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-1 Amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 210.283 µL | 1.051 mL | 2.103 mL | 10.514 mL | 21.028 mL |
5 mM | 42.057 µL | 210.283 µL | 420.566 µL | 2.103 mL | 4.206 mL |
10 mM | 21.028 µL | 105.141 µL | 210.283 µL | 1.051 mL | 2.103 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 15 references: Citation Explorer
BHQ-Cyanine-Based "Off-On" Long-Circulating Assembly as a Ferroptosis Amplifier for Cancer Treatment: A Lipid-Peroxidation Burst Device.
Authors: Sang, Mangmang and Luo, Renjie and Bai, Yidan and Dou, Jun and Zhang, Zhongtao and Liu, Fulei and Feng, Feng and Liu, Wenyuan
Journal: ACS applied materials & interfaces (2019): 42873-42884
Authors: Sang, Mangmang and Luo, Renjie and Bai, Yidan and Dou, Jun and Zhang, Zhongtao and Liu, Fulei and Feng, Feng and Liu, Wenyuan
Journal: ACS applied materials & interfaces (2019): 42873-42884
Förster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing.
Authors: Pillai, Sreenadh Sasidharan and Yukawa, Hiroshi and Onoshima, Daisuke and Biju, Vasudevanpillai and Baba, Yoshinobu
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2017): 137-142
Authors: Pillai, Sreenadh Sasidharan and Yukawa, Hiroshi and Onoshima, Daisuke and Biju, Vasudevanpillai and Baba, Yoshinobu
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2017): 137-142
The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs.
Authors: Xiang, Dongshan and Li, Fengquan and Wu, Chenyi and Shi, Boan and Zhai, Kun
Journal: Talanta (2017): 289-294
Authors: Xiang, Dongshan and Li, Fengquan and Wu, Chenyi and Shi, Boan and Zhai, Kun
Journal: Talanta (2017): 289-294
One-pot Synthesis of Quencher Labeled Hairpin DNA-CdTe QDs Conjugate for Target DNA and Deoxyribonuclease I Detection.
Authors: Zhang, Qingfeng and Li, Juanmin and Zhang, Yunyi and Hu, Zhenzhen and Yu, Cong
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2016): 1035-1037
Authors: Zhang, Qingfeng and Li, Juanmin and Zhang, Yunyi and Hu, Zhenzhen and Yu, Cong
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2016): 1035-1037
A Small-Molecule Inhibitor of Lin28.
Authors: Roos, Martina and Pradère, Ugo and Ngondo, Richard P and Behera, Alok and Allegrini, Sara and Civenni, Gianluca and Zagalak, Julian A and Marchand, Jean-Rémy and Menzi, Mirjam and Towbin, Harry and Scheuermann, Jörg and Neri, Dario and Caflisch, Amedeo and Catapano, Carlo V and Ciaudo, Constance and Hall, Jonathan
Journal: ACS chemical biology (2016): 2773-2781
Authors: Roos, Martina and Pradère, Ugo and Ngondo, Richard P and Behera, Alok and Allegrini, Sara and Civenni, Gianluca and Zagalak, Julian A and Marchand, Jean-Rémy and Menzi, Mirjam and Towbin, Harry and Scheuermann, Jörg and Neri, Dario and Caflisch, Amedeo and Catapano, Carlo V and Ciaudo, Constance and Hall, Jonathan
Journal: ACS chemical biology (2016): 2773-2781
Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated With Peptide for Biosensing Application.
Authors: Pillai, Sreenadh Sasidharan and Yukawa, Hiroshi and Onoshima, Daisuke and Biju, Vasudevanpillai and Baba, Yoshinobu
Journal: Cell medicine (2015): 57-62
Authors: Pillai, Sreenadh Sasidharan and Yukawa, Hiroshi and Onoshima, Daisuke and Biju, Vasudevanpillai and Baba, Yoshinobu
Journal: Cell medicine (2015): 57-62
Real-time fluorescence assays to monitor duplex unwinding and ATPase activities of helicases.
Authors: Özeş, Ali R and Feoktistova, Kateryna and Avanzino, Brian C and Baldwin, Enoch P and Fraser, Christopher S
Journal: Nature protocols (2014): 1645-61
Authors: Özeş, Ali R and Feoktistova, Kateryna and Avanzino, Brian C and Baldwin, Enoch P and Fraser, Christopher S
Journal: Nature protocols (2014): 1645-61
Optimal dye-quencher pairs for the design of an "activatable" nanoprobe for optical imaging.
Authors: Simard, Bryan and Tomanek, Boguslaw and van Veggel, Frank C J M and Abulrob, Abedelnasser
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2013): 1824-9
Authors: Simard, Bryan and Tomanek, Boguslaw and van Veggel, Frank C J M and Abulrob, Abedelnasser
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2013): 1824-9
Universal dark quencher based on "clicked" spectrally distinct azo dyes.
Authors: Chevalier, Arnaud and Hardouin, Julie and Renard, Pierre-Yves and Romieu, Anthony
Journal: Organic letters (2013): 6082-5
Authors: Chevalier, Arnaud and Hardouin, Julie and Renard, Pierre-Yves and Romieu, Anthony
Journal: Organic letters (2013): 6082-5
Signal-amplification detection of small molecules by use of Mg2+-dependent DNAzyme.
Authors: Guo, Zhijun and Wang, Jiahai and Wang, Erkang
Journal: Analytical and bioanalytical chemistry (2013): 4051-7
Authors: Guo, Zhijun and Wang, Jiahai and Wang, Erkang
Journal: Analytical and bioanalytical chemistry (2013): 4051-7
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?