Name: AzoDye-1 Azide
Brand: AAT Bioquest
SKU: 2412
Price: 108 USD
Availability: InStock

AzoDye-1 Azide

AzoDye-1 azide is an excellent building block for labeling alkyne-containing molecules. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2412	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-1 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	174.633 µL	873.164 µL	1.746 mL	8.732 mL	17.463 mL
5 mM	34.927 µL	174.633 µL	349.266 µL	1.746 mL	3.493 mL
10 mM	17.463 µL	87.316 µL	174.633 µL	873.164 µL	1.746 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	572.63 g/mol	=	mL	x	mM	=	mmol

References

View all 15 references: Citation Explorer

BHQ-Cyanine-Based "Off-On" Long-Circulating Assembly as a Ferroptosis Amplifier for Cancer Treatment: A Lipid-Peroxidation Burst Device.

Authors:

Sang, Mangmang and Luo, Renjie and Bai, Yidan and Dou, Jun and Zhang, Zhongtao and Liu, Fulei and Feng, Feng and Liu, Wenyuan

Journal:

ACS applied materials & interfaces (2019): 42873-42884

The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs.

Authors:

Xiang, Dongshan and Li, Fengquan and Wu, Chenyi and Shi, Boan and Zhai, Kun

Journal:

Talanta (2017): 289-294

Förster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing.

Authors:

Pillai, Sreenadh Sasidharan and Yukawa, Hiroshi and Onoshima, Daisuke and Biju, Vasudevanpillai and Baba, Yoshinobu

Journal:

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2017): 137-142

A Small-Molecule Inhibitor of Lin28.

Authors:

Roos, Martina and Pradère, Ugo and Ngondo, Richard P and Behera, Alok and Allegrini, Sara and Civenni, Gianluca and Zagalak, Julian A and Marchand, Jean-Rémy and Menzi, Mirjam and Towbin, Harry and Scheuermann, Jörg and Neri, Dario and Caflisch, Amedeo and Catapano, Carlo V and Ciaudo, Constance and Hall, Jonathan

Journal:

ACS chemical biology (2016): 2773-2781

One-pot Synthesis of Quencher Labeled Hairpin DNA-CdTe QDs Conjugate for Target DNA and Deoxyribonuclease I Detection.

Authors:

Zhang, Qingfeng and Li, Juanmin and Zhang, Yunyi and Hu, Zhenzhen and Yu, Cong

Journal:

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2016): 1035-1037

Physical properties

Molecular weight	572.63
Solvent	DMSO

Spectral properties

Absorbance (nm)	534
Extinction coefficient (cm ^-1 M ^-1)	34000

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 23, 2025