AzoDye-1 Maleimide
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 626.67 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 522 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
AzoDye-1 Succinimidyl Ester |
AzoDye-1 Amine |
AzoDye-1 Azide |
AzoDye-1 Alkyne |
Overview | SDSProtocol |
See also: Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR)
Molecular weight 626.67 | Absorbance (nm) 522 |
AzoDye-1 maleimide is an excellent building block for labeling thiol-containing molecules. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-1 Maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 159.574 µL | 797.868 µL | 1.596 mL | 7.979 mL | 15.957 mL |
5 mM | 31.915 µL | 159.574 µL | 319.147 µL | 1.596 mL | 3.191 mL |
10 mM | 15.957 µL | 79.787 µL | 159.574 µL | 797.868 µL | 1.596 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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References
View all 50 references: Citation Explorer
In Vivo Imaging of Hypoxia Associated with Inflammatory Bowel Disease by a Cytoplasmic Protein-Powered Fluorescence Cascade Amplifier.
Authors: Zhou, Yibo and Yang, Sheng and Guo, Jingru and Dong, Hao and Yin, Keyi and Huang, Wei Tao and Yang, Ronghua
Journal: Analytical chemistry (2020): 5787-5794
Authors: Zhou, Yibo and Yang, Sheng and Guo, Jingru and Dong, Hao and Yin, Keyi and Huang, Wei Tao and Yang, Ronghua
Journal: Analytical chemistry (2020): 5787-5794
Optical Supramolecular Sensing of Creatinine.
Authors: Sierra, Andrés F and Hernández-Alonso, Daniel and Romero, Miguel A and González-Delgado, José A and Pischel, Uwe and Ballester, Pablo
Journal: Journal of the American Chemical Society (2020): 4276-4284
Authors: Sierra, Andrés F and Hernández-Alonso, Daniel and Romero, Miguel A and González-Delgado, José A and Pischel, Uwe and Ballester, Pablo
Journal: Journal of the American Chemical Society (2020): 4276-4284
Preparation of antibody-immobilized gelatin nanospheres incorporating a molecular beacon to visualize the biological function of macrophages.
Authors: Yoshimoto, Yu and Jo, Jun-Ichiro and Tabata, Yasuhiko
Journal: Regenerative therapy (2020): 11-18
Authors: Yoshimoto, Yu and Jo, Jun-Ichiro and Tabata, Yasuhiko
Journal: Regenerative therapy (2020): 11-18
Quantitative Detection and Imaging of Multiple Biological Molecules in Living Cells for Cell Screening.
Authors: Huang, Shan and Zhang, Qianying and Yao, Huiqin and Wang, Wenjing and Zhang, Jian-Rong and Zhu, Jun-Jie
Journal: ACS sensors (2020): 1149-1157
Authors: Huang, Shan and Zhang, Qianying and Yao, Huiqin and Wang, Wenjing and Zhang, Jian-Rong and Zhu, Jun-Jie
Journal: ACS sensors (2020): 1149-1157
Aptamer-Functionalized Activatable DNA Tetrahedron Nanoprobe for PIWI-Interacting RNA Imaging and Regulating in Cancer Cells.
Authors: Jia, Ruichen and He, Xiaoxiao and Ma, Wenjie and Lei, Yanli and Cheng, Hong and Sun, Huanhuan and Huang, Jin and Wang, Kemin
Journal: Analytical chemistry (2019): 15107-15113
Authors: Jia, Ruichen and He, Xiaoxiao and Ma, Wenjie and Lei, Yanli and Cheng, Hong and Sun, Huanhuan and Huang, Jin and Wang, Kemin
Journal: Analytical chemistry (2019): 15107-15113
Detection of BCR-ABL1 fusion gene transcripts in the saliva of Nigerian patients with chronic myeloid leukemia.
Authors: Uzoma, I C and Taiwo, I A and Nna, E O and Durosinmi, M A and Ukaejiofo, E O
Journal: Nigerian journal of clinical practice (2019): 51-55
Authors: Uzoma, I C and Taiwo, I A and Nna, E O and Durosinmi, M A and Ukaejiofo, E O
Journal: Nigerian journal of clinical practice (2019): 51-55
Quantum Dot Nanobeacons for Single RNA Labeling and Imaging.
Authors: Ma, Yingxin and Mao, Guobin and Huang, Weiren and Wu, Guoqiang and Yin, Wen and Ji, Xinghu and Deng, Zishi and Cai, Zhiming and Zhang, Xian-En and He, Zhike and Cui, Zongqiang
Journal: Journal of the American Chemical Society (2019): 13454-13458
Authors: Ma, Yingxin and Mao, Guobin and Huang, Weiren and Wu, Guoqiang and Yin, Wen and Ji, Xinghu and Deng, Zishi and Cai, Zhiming and Zhang, Xian-En and He, Zhike and Cui, Zongqiang
Journal: Journal of the American Chemical Society (2019): 13454-13458
A carbon dot and molecular beacon based fluorometric sensor for the cancer marker microRNA-21.
Authors: Mahani, Mohamad and Mousapour, Zhahra and Divsar, Faten and Nomani, Alireza and Ju, Huangxian
Journal: Mikrochimica acta (2019): 132
Authors: Mahani, Mohamad and Mousapour, Zhahra and Divsar, Faten and Nomani, Alireza and Ju, Huangxian
Journal: Mikrochimica acta (2019): 132
Quantitative real-time PCR assay for the rapid identification of the intrinsically multidrug-resistant bacterial pathogen Stenotrophomonas maltophilia.
Authors: Fraser, Tamieka A and Bell, Mikaela G and Harris, Patrick N A and Bell, Scott C and Bergh, Haakon and Nguyen, Thuy-Khanh and Kidd, Timothy J and Nimmo, Graeme R and Sarovich, Derek S and Price, Erin P
Journal: Microbial genomics (2019)
Authors: Fraser, Tamieka A and Bell, Mikaela G and Harris, Patrick N A and Bell, Scott C and Bergh, Haakon and Nguyen, Thuy-Khanh and Kidd, Timothy J and Nimmo, Graeme R and Sarovich, Derek S and Price, Erin P
Journal: Microbial genomics (2019)
Correction: Cyclopeptidic photosensitizer prodrugs as proteolytically triggered drug delivery systems of pheophorbide A: part II - co-loading of pheophorbide A and black hole quencher.
Authors: Bouilloux, Jordan and Yushchenko, Oleksandr and Dereka, Bogdan and Boso, Gianluca and Babič, Andréj and Zbinden, Hugo and Vauthey, Eric and Lange, Norbert
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2019): 2815
Authors: Bouilloux, Jordan and Yushchenko, Oleksandr and Dereka, Bogdan and Boso, Gianluca and Babič, Andréj and Zbinden, Hugo and Vauthey, Eric and Lange, Norbert
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2019): 2815
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?