AzoDye-2 Succinimidyl Ester

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	603.59
Solvent	DMSO

Spectral properties

Absorbance (nm)

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Overview SDS Protocol

Molecular weight

603.59

Absorbance (nm)

554

AzoDye-2 Succinimidyl Ester is an excellent building block for labeling amino-containing molecules. AzoDye-2 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-2 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-2's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-2 Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	165.675 µL	828.377 µL	1.657 mL	8.284 mL	16.568 mL
5 mM	33.135 µL	165.675 µL	331.351 µL	1.657 mL	3.314 mL
10 mM	16.568 µL	82.838 µL	165.675 µL	828.377 µL	1.657 mL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)

554

Images

Figure 1. Chemical structure for AzoDye-2 Succinimidyl Ester.

References

View all 50 references: Citation Explorer

In Vivo Imaging of Hypoxia Associated with Inflammatory Bowel Disease by a Cytoplasmic Protein-Powered Fluorescence Cascade Amplifier.
Authors: Zhou, Yibo and Yang, Sheng and Guo, Jingru and Dong, Hao and Yin, Keyi and Huang, Wei Tao and Yang, Ronghua
Journal: Analytical chemistry (2020): 5787-5794

Optical Supramolecular Sensing of Creatinine.
Authors: Sierra, Andrés F and Hernández-Alonso, Daniel and Romero, Miguel A and González-Delgado, José A and Pischel, Uwe and Ballester, Pablo
Journal: Journal of the American Chemical Society (2020): 4276-4284

Preparation of antibody-immobilized gelatin nanospheres incorporating a molecular beacon to visualize the biological function of macrophages.
Authors: Yoshimoto, Yu and Jo, Jun-Ichiro and Tabata, Yasuhiko
Journal: Regenerative therapy (2020): 11-18

Quantitative Detection and Imaging of Multiple Biological Molecules in Living Cells for Cell Screening.
Authors: Huang, Shan and Zhang, Qianying and Yao, Huiqin and Wang, Wenjing and Zhang, Jian-Rong and Zhu, Jun-Jie
Journal: ACS sensors (2020): 1149-1157

BHQ-Cyanine-Based "Off-On" Long-Circulating Assembly as a Ferroptosis Amplifier for Cancer Treatment: A Lipid-Peroxidation Burst Device.
Authors: Sang, Mangmang and Luo, Renjie and Bai, Yidan and Dou, Jun and Zhang, Zhongtao and Liu, Fulei and Feng, Feng and Liu, Wenyuan
Journal: ACS applied materials & interfaces (2019): 42873-42884

Dual-Acceptor-Based Upconversion Luminescence Nanosensor with Enhanced Quenching Efficiency for in Situ Imaging and Quantification of MicroRNA in Living Cells.
Authors: Yang, Lin and Zhang, Keying and Bi, Sai and Zhu, Jun-Jie
Journal: ACS applied materials & interfaces (2019): 38459-38466

Förster Resonance Energy Transfer-Based Soft Nanoballs for Specific and Amplified Detection of MicroRNAs.
Authors: Cheng, Yun Ying and Xie, Yi Fen and Li, Chun Mei and Li, Yuan Fang and Huang, Cheng Zhi
Journal: Analytical chemistry (2019): 11023-11029

[Fluorescence assay for the detection of apurinic/apyrimidinic endonuclease 1 (APE1) activity in human blood samples].
Authors: Wang, J Y and Zhao, M P
Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (2019): 487-492

Correction: Cyclopeptidic photosensitizer prodrugs as proteolytically triggered drug delivery systems of pheophorbide A: part II - co-loading of pheophorbide A and black hole quencher.
Authors: Bouilloux, Jordan and Yushchenko, Oleksandr and Dereka, Bogdan and Boso, Gianluca and Babič, Andréj and Zbinden, Hugo and Vauthey, Eric and Lange, Norbert
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2019): 2815

Quantitative real-time PCR assay for the rapid identification of the intrinsically multidrug-resistant bacterial pathogen Stenotrophomonas maltophilia.
Authors: Fraser, Tamieka A and Bell, Mikaela G and Harris, Patrick N A and Bell, Scott C and Bergh, Haakon and Nguyen, Thuy-Khanh and Kidd, Timothy J and Nimmo, Graeme R and Sarovich, Derek S and Price, Erin P
Journal: Microbial genomics (2019)