AzoDye-2 Succinimidyl Ester

AzoDye-2 Succinimidyl Ester is an excellent building block for labeling amino-containing molecules. AzoDye-2 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-2 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-2's ability to form stable, non-fluorescent complexes with a fluorophore.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2422	5 mg	Price

References

View all 50 references: Citation Explorer

In Vivo Imaging of Hypoxia Associated with Inflammatory Bowel Disease by a Cytoplasmic Protein-Powered Fluorescence Cascade Amplifier.

Authors:

Zhou, Yibo and Yang, Sheng and Guo, Jingru and Dong, Hao and Yin, Keyi and Huang, Wei Tao and Yang, Ronghua

Journal:

Analytical chemistry (2020): 5787-5794

Optical Supramolecular Sensing of Creatinine.

Authors:

Sierra, Andrés F and Hernández-Alonso, Daniel and Romero, Miguel A and González-Delgado, José A and Pischel, Uwe and Ballester, Pablo

Journal:

Journal of the American Chemical Society (2020): 4276-4284

Preparation of antibody-immobilized gelatin nanospheres incorporating a molecular beacon to visualize the biological function of macrophages.

Authors:

Yoshimoto, Yu and Jo, Jun-Ichiro and Tabata, Yasuhiko

Journal:

Regenerative therapy (2020): 11-18

Quantitative Detection and Imaging of Multiple Biological Molecules in Living Cells for Cell Screening.

Authors:

Huang, Shan and Zhang, Qianying and Yao, Huiqin and Wang, Wenjing and Zhang, Jian-Rong and Zhu, Jun-Jie

Journal:

ACS sensors (2020): 1149-1157

BHQ-Cyanine-Based "Off-On" Long-Circulating Assembly as a Ferroptosis Amplifier for Cancer Treatment: A Lipid-Peroxidation Burst Device.

Authors:

Sang, Mangmang and Luo, Renjie and Bai, Yidan and Dou, Jun and Zhang, Zhongtao and Liu, Fulei and Feng, Feng and Liu, Wenyuan

Journal:

ACS applied materials & interfaces (2019): 42873-42884

Physical properties

Molecular weight	603.59
Solvent	DMSO

Spectral properties

Absorbance (nm)

554

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025